Anti-MOX1/MEOX1抗体(ab125712)
Key features and details
- Rabbit polyclonal to MOX1/MEOX1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MOX1/MEOX1抗体
参阅全部 MOX1/MEOX1 一抗 -
描述
兔多克隆抗体to MOX1/MEOX1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Macaque monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide within Human MOX1/MEOX1 aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P50221 -
阳性对照
- This antibody gave a positive signal in Human Fetal Heart tissue lysate. WB: Human liver cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with abX overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406 -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125712于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 27 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 27 kDa). |
靶标
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功能
Mesodermal transcription factor that plays a key role in somitogenesis and is specifically required for sclerotome development. Required for maintenance of the sclerotome polarity and formation of the cranio-cervical joints (PubMed:23290072, PubMed:24073994). Binds specifically to the promoter of target genes and regulates their expression. Activates expression of NKX3-2 in the sclerotome. Activates expression of CDKN1A and CDKN2A in endothelial cells, acting as a regulator of vascular cell proliferation. While it activates CDKN1A in a DNA-dependent manner, it activates CDKN2A in a DNA-independent manner. Required for hematopoietic stem cell (HSCs) induction via its role in somitogenesis: specification of HSCs occurs via the deployment of a specific endothelial precursor population, which arises within a sub-compartment of the somite named endotome. -
疾病相关
Klippel-Feil syndrome 2, autosomal recessive -
序列相似性
Contains 1 homeobox DNA-binding domain. -
细胞定位
Nucleus. Cytoplasm. Localizes predominantly in the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4222 Human
- Omim: 600147 Human
- SwissProt: P50221 Human
- Unigene: 438 Human
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别名
- Homeobox protein MOX-1 antibody
- MEOX1 antibody
- MEOX1_HUMAN antibody
see all
图片
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All lanes : Anti-MOX1/MEOX1 antibody (ab125712) at 1 µg/ml
Lane 1 : Human liver cell lysate
Lane 2 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-MOX1/MEOX1 antibody staining at 1 ug/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab125712 was shown to bind specifically to MOX1/MEOX1. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Anti-MOX1/MEOX1 antibody (ab125712) at 1 µg/ml + Heart (Human) Whole Cell Lysate - fetal normal tissue at 25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa (possible non-specific binding), 75 kDa (possible non-specific binding)
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab125712 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
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All lanes : Anti-MOX1/MEOX1 antibody (ab125712) at 1 µg/ml
Lane 1 : Heart (Human) Whole Cell Lysate - fetal normal tissue
Lane 2 : Heart (Human) Whole Cell Lysate - fetal normal tissue with Immunising peptide at 1 µg/ml
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa (possible non-specific binding), 65 kDa (possible non-specific binding)
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab125712 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab125712 尚未被引用在任何文献中。