概述

  • 产品名称Anti-Mov10抗体
    参阅全部 Mov10 一抗
  • 描述
    兔多克隆抗体to Mov10
  • 经测试应用适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 953 and 1003 of human Mov10 homolog (NP_066014.1)

  • 阳性对照
    • HeLa, 293T and NIH3T3 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab80613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 114 kDa.
IP Use at 2-5 µg/mg of lysate.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 相关性MOV10 may be an helicase with an important function in development and/or control of cell proliferation. RNA silencing processes are guided by small RNAs known as siRNAs and microRNAs (miRNAs). They reside in ribonucleoprotein complexes, which guide the cleavage of complementary mRNAs or affect stability and translation of partial complementary mRNAs. Argonaute (Ago) proteins are at the heart of silencing effector complexes and bind the single-stranded siRNA and miRNA. Ago1- and Ago2-containing complexes have been purified from human cells, resulting in the discovery of novel factors such as the putative RNA helicase MOV10, and the RNA recognition motif (RRM)-containing protein TNRC6B/KIAA1093. The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • DKFZp667O1423 antibody
    • FLJ32791 antibody
    • fSAP113 antibody
    • Functional spliceosome-associated protein 113 antibody
    • gb110 antibody
    • MGC2948 antibody
    • Moloney leukemia virus 10 protein antibody
    • MOV 10 antibody
    • Mov10 (Moloney leukemia virus 10, mouse) homolog antibody
    • Mov10 (Moloney leukemia virus 10,) homolog antibody
    • Mov10, Moloney leukemia virus 10, homolog (mouse) antibody
    • Mov10, Moloney leukemia virus 10, homolog antibody
    • Putative helicase MOV10 antibody
    see all

Anti-Mov10 antibody 图像

  • All lanes : Anti-Mov10 antibody (ab80613) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 114 kDa
    Observed band size : 114 kDa


    Exposure time : 3 minutes
  • Immunoprecipitation of HeLa whole cell lysate using ab80613 at 3µg/mg (lane 1) or control IgG (lane 2). Samples were analysed by Western blot using ab80613 at 1µg/ml.
    Detection: Chemiluminescence with an exposure time of 30 seconds.
  • ICC/IF image of ab80613 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80613, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Mov10 antibody (ab80613)参考文献

This product has been referenced in:

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% triton x100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
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提交于 Sep 19 2012

Thank you for contacting us.

I am sorry to hear of the troubles which you have been experiencing using our products. Could please provide me with details of your western blotting protocol as well as tissue type and preparation steps and an...

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Thank you for your inquiry. Unfortunately, we do not know of a publication where this antibody was used in a co-IP experiment. I would like to reassure you that this antibody is tested and guaranteed for IP by our Abpromise. To co-IP a protei...

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