小鼠Werner's syndrome helicase WRN肽(ab19370)

概述

描述

  • 性质Synthetic

技术指标

Our Abpromise guarantee covers the use of ab19370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Blocking - Blocking peptide for Anti-Werner's syndrome helicase WRN antibody (ab16489)

  • 形式Liquid
  • 补充说明

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

常规信息

  • 别名
    • DKFZp686C2056
    • DNA helicase
    • DNA helicase, RecQ like type 3
    • Exonuclease WRN
    • HGNC 12791
    • OTTHUMP00000225301
    • RecQ protein-like 2
    • RecQ-like type 3
    • RecQ3
    • RECQL2
    • RECQL3
    • Werner syndrome ATP-dependent helicase
    • Werner syndrome helicase
    • Werner syndrome protein
    • Werner syndrome, RecQ helicase like
    • WRN
    • WRN_HUMAN
    see all
  • 功能Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A.
  • 疾病相关Defects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.
    Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:114500].
  • 序列相似性Belongs to the helicase family. RecQ subfamily.
    Contains 1 3'-5' exonuclease domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.
  • 翻译后修饰Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus > nucleolus. Nucleus.
  • Information by UniProt

Mouse Werner's syndrome helicase WRN peptide 图像

  • All lanes : Anti-Werner's syndrome helicase WRN antibody (ab16489) at 1 µg/ml

    Lane 1 : Mouse testis lysate
    Lane 2 : Mouse testes lysate with Mouse Werner's syndrome helicase WRN peptide (ab19370) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilution

    Predicted band size : 162 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)

Mouse Werner's syndrome helicase WRN peptide (ab19370)参考文献

ab19370 has not yet been referenced specifically in any publications.

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