小鼠VEGFA ELISA试剂盒(ab119565)

概述

  • 产品名称
    小鼠VEGFA ELISA试剂盒
    参阅全部 VEGFA 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 6 3.8%
    批次间
    样品 n Mean SD CV%
    Overall 6 6.4%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    20 pg/ml
  • 范围
    62.5 pg/ml - 4000 pg/ml
  • 复检

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 75% - 97%
    Serum 87% - 95%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s VEGFA Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse VEGFA in cell culture supernatant, serum and plasma (EDTA).


    Pay attention to a possibly elevated serum level of mouse VEGFA due to VEGFA release by platelets during platelet activation (sampling process).


    VEGFA specific antibodies have been precoated onto 96-well plates. Standards and samples are pipetted into the wells and VEGFA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse VEGFA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGFA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Mouse VEGF ELISA Kit (ab209882) from our SimpleStep ELISA® range.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X conjugate diluent 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 6 units
    Assay Diluent 1 x 12ml
    Biotin-Conjugate anti-mouse VEGFA polyclonal antibody 1 x 120µl
    Microplate coated with polyclonal antibody to Mouse VEGFA (12 x 8 wells) 1 unit
    Sample Diluent 1 x 10ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
    VEGFA Standard lyophilized 2 vials
  • 研究领域
  • 功能
    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • 组织特异性
    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • 疾病相关
    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • 序列相似性
    Belongs to the PDGF/VEGF growth factor family.
  • 细胞定位
    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt
  • 别名
    • Vascular endothelial growth factor A
    • Vascular permeability factor
    • VEGF-A
    • Vegfa
    • VEGFA_HUMAN
    • VPF
    see all
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab119565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab119565.

实验方案

文献

ab119565 has not yet been referenced specifically in any publications.

客户评价及客户问答

nice kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews
kit worked, easy to use. attached is the data for standard curve. I used cell media from 12 well plate, the media were not concentrated nor diluted, it was directly added to the wells of the kit.
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提交于 Feb 07 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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