小鼠VE Cadherin ELISA试剂盒(ab206980)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 167.6 pg/ml
- Range: 234.37 pg/ml - 15000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
-
产品名称
小鼠VE Cadherin ELISA试剂盒
参阅全部 VE Cadherin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5 4.7% 批次间 样品 n Mean SD CV% Overall 3 6.6% -
样品类型
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
167.6 pg/ml -
范围
234.37 pg/ml - 15000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 107 107% - 109% Tissue Culture Media 103 101% - 104% Hep Plasma 95 86% - 100% EDTA Plasma 99 94% - 103% Cit plasma 106 105% - 109% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Mouse -
产品概述
Mouse VE Cadherin ELISA Kit (ab206980) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VE Cadherin protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse VE Cadherin with 167.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
说明
VE-Cadherin (also known as Cadherin 5 or CD144) is a calcium-dependent cell-cell adhesion glycoprotein. VE-Cadherin is a member of the cadherin superfamily, which interact with themselves in connecting cells. VE-Cadherin is expressed in endothelial cells and interacts with KRIT1 to establish and maintain endothelial polarity.
-
平台
Microplate (12 x 8 well strips)
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Mouse VE-Cadherin Capture Antibody 1 x 600µl 10X Mouse VE-Cadherin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse VE-Cadherin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
-
功能
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. -
组织特异性
Endothelial tissues and brain. -
序列相似性
Contains 5 cadherin domains. -
翻译后修饰
Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB. -
细胞定位
Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries. - Information by UniProt
-
别名
- 7B 4
- 7B4
- 7B4 antigen
see all -
数据库链接
- Entrez Gene: 12562 Mouse
- SwissProt: P55284 Mouse
- Unigene: 21767 Mouse
相关产品
-
Alternative Versions
图片
-
Background-subtracted data values (mean +/- SD) are graphed.
-
The concentrations of mouse VE-Cadherin were measured in duplicate and interpolated from the mouse VE-Cadherin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD). 1X serum is at a concentration of 0.25%, and 1X plasma (citrate and EDTA) is at a concentration of 0.5%, and 1X plasma (heparin) is at a concentration of 0.25%.
-
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
To learn more about the advantages of recombinant antibodies see here.
-
To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab206980 尚未被引用在任何文献中。