小鼠Tweak ELISA试剂盒(ab113320)

概述

  • 产品名称小鼠Tweak ELISA试剂盒
    参阅全部 Tweak 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 0.3 pg/ml
  • 范围
    0.27 pg/ml - 200 pg/ml
  • 复检

    77 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 75.69 68% - 83%
    Serum 79.66 71% - 87%
    Plasma 77.72 70% - 89%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s Tweak Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Tweak in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Tweak coated on a 96-well plate. Standards and samples are pipetted into the wells and Tweak present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Tweak antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Tweak bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

应用

Our Abpromise guarantee covers the use of ab113320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse Tweak ELISA Kit 图像

  • Representative Standard Curve using ab113320.

实验方案

Mouse Tweak ELISA Kit (ab113320)参考文献

ab113320 has not yet been referenced specifically in any publications.

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This ELISA kit has not been tested against monomeric TWEAK/TNFSF12. It is only known to detect mouse TWEAK in its trimeric form. It is important to note that TWEAK is a homotrimer, mean...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"