小鼠TrkB ELISA试剂盒(ab119610)

概述

  • 产品名称小鼠TrkB ELISA试剂盒
    参阅全部 TrkB 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Cell Lysate, Tissue Homogenate
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 5 pg/ml
  • 范围
    93.8 pg/ml - 6000 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s mouse TrkB in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Mouse TrkB in cell culture supernatants, cell lysates and tissue homogenates.

    A TrkB specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for TrkB is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse TrkB amount of sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
  • 组织特异性Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Ligand-mediated auto-phosphorylation.
  • 细胞定位Membrane.
  • Information by UniProt
  • 别名
    • BDNF tropomyosine receptor kinase B
    • BDNF/NT-3 growth factors receptor
    • GP145-TrkB
    • Neurotrophic tyrosine kinase receptor type 2
    • Ntrk2
    • NTRK2_HUMAN
    • Trk-B
    • TRKB
    • TrkB tyrosine kinase
    • Tyrosine kinase receptor B
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse TrkB ELISA Kit 图像

  • Representative standard curve using ab119610

实验方案

Mouse TrkB ELISA Kit (ab119610)参考文献

ab119610 has not yet been referenced specifically in any publications.

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Unfortunately, this kit cannot be used with rat samples.

We have 2 TrkB antibodies (ab51190 and ab43079) that have been tested in indirect ELISA and rat samples, and might work together in a sandwich EL...

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DISCOUNT CODE: ***
Expiration date: June 15th, 2012

I am very pleased to hear you would like to accept our offer and test ab119609 in mouse. This code will give you 1 free ELISA kit before the expiration date. To redeem this offer, pleas...

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The lab informed me thatRIPA buffer is compatible with the kit and can be usedwithtissue.

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