概述

  • 产品名称
    小鼠TNF alpha ELISA试剂盒
    参阅全部 TNF alpha 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    A 8 553.5pg/ml 11.2 2%
    B 8 124pg/ml 6.4 5.1%
    批次间
    样品 n Mean SD CV%
    A 10 528.7pg/ml 37.3 7%
    B 10 132.2pg/ml 10.8 8.1%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 25 pg/ml
  • 范围
    31.25 pg/ml - 1000 pg/ml
  • 检测时间
    3h 45m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s TNF alpha mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TNF alpha in mouse serum, buffered solutions and cell culture medium.

    A monoclonal antibody specific for TNF alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TNF alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TNF alpha present in the samples.

    This kit will recognize both endogenous and recombinant mouse TNF alpha.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 标识符 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-TNFalpha Red 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Plastic Plate Covers 1 x 2 units 2 x 2 units
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
    TNF alpha Microplate (12 x 8 well strips) 1 unit 2 units
    TNF alpha Standard (lyophilized) Yellow 2 vials 4 vials
  • 研究领域
  • 功能
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • 疾病相关
    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • 序列相似性
    Belongs to the tumor necrosis factor family.
  • 翻译后修饰
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • 细胞定位
    Secreted and Cell membrane.
  • Information by UniProt
  • 别名
    • APC1 protein
    • Cachectin
    • DIF
    • soluble form
    • Tnf
    • TNF a
    • TNF macrophage derived
    • TNF monocyte derived
    • TNF superfamily member 2
    • TNF-a
    • TNF-alpha
    • TNFA_HUMAN
    • TNFSF2
    • Tumor necrosis factor
    • Tumor necrosis factor alpha
    • Tumor necrosis factor ligand superfamily member 2
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab46105

实验方案

文献

This product has been referenced in:
  • Al-Muzafar HM & Amin KA Probiotic mixture improves fatty liver disease by virtue of its action on lipid profiles, leptin, and inflammatory biomarkers. BMC Complement Altern Med 17:43 (2017). Rat . Read more (PubMed: 28086768) »
  • Yang X  et al. Exposure to excess insulin (glargine) induces type 2 diabetes mellitus in mice fed on a chow diet. J Endocrinol 221:469-80 (2014). Sandwich ELISA ; Mouse . Read more (PubMed: 24741073) »

See all 2 Publications for this product

客户评价及客户问答

The ELISA assays are compatible with RIPA buffer. However, you should prepare your standards and positive controls in the same buffer.

The Bradford assay is compatible with RIPA buffer if the samples are diluted at least 10-fold with distill...

Read More

Thank you for your inquiry. Yes, this kit can be used on tissue lysates, but your samples may need to be dialyzed in PBS before being assayed. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more a...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册