小鼠TIMP2 ELISA试剂盒(ab100746)

概述

  • 产品名称小鼠TIMP2 ELISA试剂盒
    参阅全部 TIMP2 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 80 pg/ml
  • 范围
    0.082 ng/ml - 60 ng/ml
  • 复检

    93 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 95.36 82% - 105%
    Serum 92.65 80% - 112%
    Plasma 93.42 81% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam's TIMP2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse TIMP2 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse TIMP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse TIMP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Matrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. The activation and activity of MMPs are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP2 (also called CSC-21K) is a 24 kDa glycoprotein that is expressed by a variety of cell types. It forms a non covalent, stoichiometric complex with both latent and active MMPs. TIMP2 shows a preference for MMP2. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models. TIMP2 is constitutively produced in low (pg/ml) levels by most cell types in tissue culture. The low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.
  • 细胞定位Secreted
  • 别名
    • CSC 21K
    • CSC21K
    • D11Bwg1104e
    • DDC8
    • Metalloproteinase inhibitor 2
    • Metalloproteinase inhibitor 2 precursor
    • MGC105282
    • OTTMUSP00000004170
    • OTTMUSP00000004171
    • RP23-394O9.2
    • TIMP 2
    • TIMP metallopeptidase inhibitor 2
    • Tissue inhibitor of metalloproteinase 2
    • Tissue inhibitor of metalloproteinases 2
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse TIMP2 ELISA Kit 图像

  • Representative Standard Curve using ab100746

  • Representative Standard Curve using ab100746

实验方案

Mouse TIMP2 ELISA Kit (ab100746)参考文献

ab100746 has not yet been referenced specifically in any publications.

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We recommend a 2 fold dilution for plasma samples for use with all three of the mouse ELISA kits.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"