概述

  • 产品名称小鼠TGF beta 1 ELISA试剂盒
    参阅全部 TGF beta 1 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4 7.9%
    批次间
    样品 n Mean SD CV%
    Overall 4 5.8%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    7.8 pg/ml
  • 范围
    31.3 pg/ml - 2000 pg/ml
  • 复检

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 124% - 141%
    Serum 92% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s TGF beta 1 Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Mouse TGF beta 1 concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate).

    Mouse TGF beta 1 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Mouse TGF beta 1 detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after the addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mouse TGF beta 1 captured on the plate

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • 组织特异性Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • 疾病相关Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • 序列相似性Belongs to the TGF-beta family.
  • 翻译后修饰Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 别名
    • Camurati-Engelmann disease
    • CED
    • DPD1
    • LAP
    • Latency-associated peptide
    • TGF beta 1 protein
    • TGF-beta-1
    • TGFB
    • tgfb1
    • TGFB1_HUMAN
    • TGFbeta
    • Transforming growth factor beta 1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse TGF beta 1 ELISA Kit 图像

  • Standard curve of Ms TGFb, with background signal subtracted (duplicates; +/- SD).
  • Mouse TGFb measured in activated biological fluids, background signal subtracted (duplicates +/- SD). * Samples without activation.
  • Representative Standard Curve using ab119557.

实验方案

Mouse TGF beta 1 ELISA Kit (ab119557)参考文献

This product has been referenced in:
  • Strijbis K  et al. Intestinal colonization by Candida albicans alters inflammatory responses in Bruton's tyrosine kinase-deficient mice. PLoS One 9:e112472 (2014). Mouse . Read more (PubMed: 25379804) »
  • Fei AH  et al. Salvianolate inhibits reactive oxygen species production in H(2)O(2)-treated mouse cardiomyocytes in vitro via the TGFß pathway. Acta Pharmacol Sin 34:496-500 (2013). Read more (PubMed: 23524570) »

See all 3 Publications for this product

Product Wall


ab108792: We have never tested brain tissue supernatants so I do not know if it would work. I cannot recommend the dilution range.

ab119557: Again, we have not tested brain tissue with this kit but there is a publication where a FlowCyto...

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Cross-reactivity with human TGF-b1

Inconclusive Average 3/5 (Ease of Use)
Abreviews
As we plan to do TGF-b1 determinations from supernatants of human and mice co-cultured cells, we checked the ability of this Kit to distinguish both forms, human and rodent.

We measured samples of DMEM, DMEM 10% and 0,5% FBS and DMEM + purified hTGF-b1 protein. Although samples with low FBS gives us a low value of TGF, samples with hTGF-b1 gives very strong signal.

We consider that specificity of the kit is not promised, so we want a discount check for the value of the Kit. Also we want to notice that we buy two different Kit to be absolute sure that the Kit is the problem not our manipulation, so we claim for both Kits...



Despite of the Kit is very easy to use, we consider that detection is a long process due to a lot steps of washing comparing to hTGFb1 detection Kit.
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Abcam user community

Verified customer

提交于 Oct 28 2013






We do have reports concerning tissue samples for ab119557 indicating that lysis buffers containing 0.5% TritonX-100 (detergent) had no deleterious effect, but we did not test this ourselves.


We also have not tes...

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Thank you for contacting us.

The TGF beta 1 Mouse ELISA Kit (with color giving dyes) (ab119557) is the only kit we currently have which can be used to detect TGF beta 1 in mouse samples. Regrettably we have not as yet tested this kit with tis...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"