小鼠TECK ELISA试剂盒(ab100743)
Key features and details
- Sensitivity: 15 pg/ml
- Range: 20.5 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
-
产品名称
小鼠TECK ELISA试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 15 pg/ml -
范围
20.5 pg/ml - 2000 pg/ml -
回收率
94 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 97.42 84% - 107% Serum 94.26 82% - 105% Plasma 92.68 80% - 106% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s TECK Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TECK in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse TECK coated on a 96- well plate. Standards and samples are pipetted into the wells and TECK present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse TECK antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TECK bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 120X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse TECK 2 vials Recombinant Mouse TECK Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TECK Microplate (12 x 8 wells) 1 unit TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
-
相关性
This gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. TECK displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. TECK binds to chemokine receptor CCR9. Two alternatively spliced variants encoding two different isoforms have been identified. -
别名
- C C motif chemokine 25
- CCL 25 MGC125074
- CCL25
see all -
数据库链接
- SwissProt: O35903 Mouse
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab100743 尚未被引用在任何文献中。