小鼠spleen组织裂解液- total protein (14 days) (ab7274)

概述

  • 产品名称
    小鼠spleen组织裂解液- total protein (14 days)
    参阅全部 Spleen 细胞裂解液
  • 描述
    小鼠spleen总蛋白裂解液
  • 常规说明

    Source: Outbred NIH-Swiss Albino mice

    The mouse spleen tissue was collected from fourteen (14) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris -HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • 经测试应用
    适用于: WBmore details

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: None
    Constituents: 5% Beta mercaptoethanol, 12.5% Glycerol, 1% SDS, 1% Triton-X-100, 1% Sodium Deoxycholate, 0.01% Bromophenol blue, 5µg/ml Leupeptin, 5µg/ml Aprotinin, 50mM Tris HCl, 150mM Sodium chloride, 1mM EDTA, 1mM PMSF, pH 6.8
  • Concentration information loading...
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Mouse spleen tissue lysate is ready to load on SDS-PAGE for Western blotting. It is recommended to load 10 µg to 20 µg per lane for mini gel.

文献

ab7274 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your enquiry. I have been in contact with the originator of this antibody, and they used mouse spleen from an adult Balb/c, approximately 6 months old. With that knowledge, I would recommend the 14 day old lysate over the 7 day old lysate...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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