小鼠spleen组织裂解液- total protein (14 days) (ab7274)

概述

  • 产品名称小鼠spleen组织裂解液- total protein (14 days)
    参阅全部 Spleen 细胞裂解液
  • 描述
    小鼠spleen总蛋白裂解液
  • 常规说明

    Source: Outbred NIH-Swiss Albino mice

    The mouse spleen tissue was collected from fourteen (14) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris -HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • 经测试应用适用于: WBmore details

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: 5% Beta mercaptoethanol, 12.5% Glycerol, 1% SDS, 1% Triton-X-100, 1% Sodium Deoxycholate, 0.01% Bromophenol blue, 5µg/ml Leupeptin, 5µg/ml Aprotinin, 50mM Tris HCl, 150mM Sodium chloride, 1mM EDTA, 1mM PMSF, pH 6.8
  • Concentration information loading...
  • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab7274 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use at an assay dependent concentration. Mouse spleen tissue lysate is ready to load on SDS-PAGE for Western blotting. It is recommended to load 10 µg to 20 µg per lane for mini gel.

    Mouse spleen tissue lysate - total protein (14 days) (ab7274)参考文献

    ab7274 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. I have been in contact with the originator of this antibody, and they used mouse spleen from an adult Balb/c, approximately 6 months old. With that knowledge, I would recommend the 14 day old lysate over the 7 day old lysate...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"