Anti-Mouse Serum Albumin抗体(HRP) (ab19195)
- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
- 产品名称Anti-Mouse Serum Albumin抗体(HRP)
参阅全部 Mouse Serum Albumin 一抗 - 描述山羊多克隆抗体to小鼠Serum Albumin (HRP)
- 偶联物HRP
- 经测试应用适用于: ELISA, WB, ICC, IHC-Pmore details
- 种属反应性与反应: Mouse
- 免疫原
Full length protein (Mouse).
- 阳性对照
- Liver (Human and mouse)
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C.
- 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.1M Sodium chloride, 50mM HEPES. pH 7.1 -
Concentration information loading... - 纯度Immunogen affinity purified
- 纯化说明Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose and conjugated to horseradish peroxidase (HRP).
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
- Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
应用
Our Abpromise guarantee covers the use of ab19195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | 1/10000 - 1/100000. | |
| WB | 1/1000 - 1/30000. Predicted molecular weight: 69 kDa. | |
| ICC | 1/200 - 1/500. | |
| IHC-P | Use at an assay dependent dilution. |
靶标
- 功能Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
- 组织特异性Plasma.
- 序列相似性Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. - 翻译后修饰Phosphorylation sites are present in the extracelllular medium.
- 细胞定位Secreted.
- Information by UniProt
-
数据库链接
- Entrez Gene: 11657 Mouse
- SwissProt: P07724 Mouse
- Unigene: 16773 Mouse
-
别名
- Alb 1 antibody
- alb antibody
- ALBU_MOUSE antibody
see all
Anti-Mouse Serum Albumin antibody (HRP) 图像
-
Western blot - Anti-Mouse Serum Albumin antibody (HRP) (ab19195)All lanes : Anti-Mouse Serum Albumin antibody (HRP) (ab19195) at 1 µg/ml
Lane 1 :Human liver tissue lysate - total protein (ab29889)
Lane 2 : Liver (Mouse) Tissue Lysate (ab7935)
Lysates/proteins at 10 µg per lane.
Secondary
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 69 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Exposure time : 10 seconds
Anti-Mouse Serum Albumin antibody (HRP) (ab19195)参考文献
This product has been referenced in:
- Liu H et al. The FGL2-FcgammaRIIB pathway: a novel mechanism leading to immunosuppression. Eur J Immunol 38:3114-26 (2008). Read more (PubMed: 18991288) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"