Anti-Mouse Serum Albumin抗体(HRP) (ab19195)

概述

  • 产品名称Anti-Mouse Serum Albumin抗体(HRP)
    参阅全部 Mouse Serum Albumin 一抗
  • 描述
    山羊多克隆抗体to小鼠Serum Albumin (HRP)
  • 偶联物HRP
  • 经测试应用适用于: ELISA, WB, ICC, IHC-Pmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Full length protein (Mouse).

  • 阳性对照
    • Liver (Human and mouse)

性能

应用

Our Abpromise guarantee covers the use of ab19195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000 - 1/100000.
WB 1/1000 - 1/30000. Predicted molecular weight: 69 kDa.
ICC 1/200 - 1/500.
IHC-P Use at an assay dependent dilution.

靶标

  • 功能Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • 组织特异性Plasma.
  • 序列相似性Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 翻译后修饰Phosphorylation sites are present in the extracelllular medium.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alb 1 antibody
    • alb antibody
    • ALBU_MOUSE antibody
    • Albumin 1 antibody
    • Albumin antibody
    • Serum albumin antibody
    see all

Anti-Mouse Serum Albumin antibody (HRP) 图像

  • All lanes : Anti-Mouse Serum Albumin antibody (HRP) (ab19195) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Mouse) Tissue Lysate (ab7935)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 69 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds

Anti-Mouse Serum Albumin antibody (HRP) (ab19195)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Thank you for contacting us.
As we haven't tested the cross-reactivity for this antibodies, it is very much likely that they will react with other albumins.
Therefore, I would suggest to use milk as a blocking agent (0.5% Skim milk/PBS for 1 h...

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Thank you for contacting us. Serum albumin may be a suitable loading control, but I am not sure if levels will vary from one animal to another, for example healthy vs. diseased. These three antibodies detect mouse serum albumin: ab19194...

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Thank you for contacting us. Feel free to call us any time between 8am and 8pm EST at 888-77-ABCAM. I am not sure what you mean by detecting the antibody on a nanofiber film. Is this film a matrix for growing cells? If so, you could use an ...

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