抗小鼠Serum Albumin抗体(HRP) (ab19195)

Submit an Abreview Q&A (3)Specific References (5)

概述

  • 产品名称
    抗小鼠Serum Albumin抗体(HRP)
    参阅全部 Mouse Serum Albumin 一抗
  • 描述
    山羊多克隆抗体to小鼠Serum Albumin (HRP)
  • 宿主
    Goat
  • 偶联物
    HRP
  • 经测试应用
    适用于: ELISA, WB, ICC, IHC-Pmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Full length protein (Mouse).

  • 阳性对照
    • Liver (Human and mouse)

性能

应用

Our Abpromise guarantee covers the use of ab19195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000 - 1/100000.
WB 1/1000 - 1/30000. Predicted molecular weight: 69 kDa.
ICC 1/200 - 1/500.
IHC-P Use at an assay dependent dilution.

靶标

  • 功能
    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • 组织特异性
    Plasma.
  • 序列相似性
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 翻译后修饰
    Phosphorylation sites are present in the extracelllular medium.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
    • 别名
      • Alb 1 antibody
      • alb antibody
      • ALBU_MOUSE antibody
      • Albumin 1 antibody
      • Albumin antibody
      • Serum albumin antibody
      see all

    图片

    • Western blot - Anti-Mouse Serum Albumin antibody (HRP) (ab19195)
      Western blot - Anti-Mouse Serum Albumin antibody (HRP) (ab19195)
      All lanes : Anti-Mouse Serum Albumin antibody (HRP) (ab19195) at 1 µg/ml

      Lane 1 : Human liver tissue lysate - total protein (ab29889)
      Lane 2 : Liver (Mouse) Tissue Lysate (ab7935)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 69 kDa
      Observed band size: 70 kDa (why is the actual band size different from the predicted?)


      Exposure time: 10 seconds

    文献

    This product has been referenced in:
    • Grevys A  et al. A human endothelial cell-based recycling assay for screening of FcRn targeted molecules. Nat Commun 9:621 (2018). Read more (PubMed: 29434196) »
    • Swiercz R  et al. Loss of expression of the recycling receptor, FcRn, promotes tumor cell growth by increasing albumin consumption. Oncotarget 8:3528-3541 (2017). Read more (PubMed: 27974681) »

    See all 5 Publications for this product

    发表研究结果有使用 ab19195？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    3 reviews or Q&A

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    Votes: Highest First

    Our customer is interested in using one of the following antibodies for the detection of albmin in mouse serum by ELISA.

    http://www.abcam.com/Mouse-Serum-Albumin-antibody-ab19194.html
    http://www.abcam.com/Mouse-Serum-Albumin-antibody-HRP-a...

    Read More

    Thank you for contacting us.
    As we haven't tested the cross-reactivity for this antibodies, it is very much likely that they will react with other albumins.
    Therefore, I would suggest to use milk as a blocking agent (0.5% Skim milk/PBS for 1 h...

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    I would like to know what antibodies you have available as Western Blot loading controls for mouse serum. Antibodies against general mouse Ig have been moderately successful but we would like to consider other options.

    Thank you for contacting us. Serum albumin may be a suitable loading control, but I am not sure if levels will vary from one animal to another, for example healthy vs. diseased. These three antibodies detect mouse serum albumin: ab19194...

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    I am using your product ab19195 and ab3781.I am going to see the antibody (ab3781) load on to a nanofibre film. I have done ELISA but here i am going to use A 1cm*1cm nanofer film instead of ELISA plate, so would please help me how I can do this and me...

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    Thank you for contacting us. Feel free to call us any time between 8am and 8pm EST at 888-77-ABCAM. I am not sure what you mean by detecting the antibody on a nanofiber film. Is this film a matrix for growing cells? If so, you could use an ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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