Anti-Mouse Serum Albumin抗体(ab34807)

概述

  • 产品名称
    Anti-Mouse Serum Albumin抗体
    参阅全部 Mouse Serum Albumin 一抗
  • 描述
    兔多克隆抗体to小鼠Serum Albumin
  • 经测试应用
    适用于: WB, IP, Immunoelectrophoresis, ELISAmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Full length native protein (purified): Mouse Serum Albumin

性能

应用

Our Abpromise guarantee covers the use of ab34807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/20000 - 1/100000.
IP Use at an assay dependent concentration.
Immunoelectrophoresis Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000. This product has been assayed against 1.0 µg of Mouse Serum Albumin in a standard indirect ELISA using Peroxidase conjugated Affinity Purified Donkey anti-Rabbit IgG and TMB as a substrate for 30 minutes at room temperature.

靶标

  • 功能
    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • 组织特异性
    Plasma.
  • 序列相似性
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 翻译后修饰
    Phosphorylation sites are present in the extracelllular medium.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alb 1 antibody
    • alb antibody
    • ALBU_MOUSE antibody
    • Albumin 1 antibody
    • Albumin antibody
    • Serum albumin antibody
    see all

图片

  • Anti-Mouse Serum Albumin antibody (ab34807) + mouse serum albumin at 0.1 µg

    Observed band size : 72 kDa (why is the actual band size different from the predicted?)

    Primary antibody: MSA antibody at 1:1000 in 1% BSA-TTBS for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:20,000 for 45 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting  30 min at 4°C.

  • All lanes : Anti-Mouse Serum Albumin antibody (ab34807)

    Lane 1 : mouse serum albumin reduced
    Lane 3 : mouse serum albumin non-reduced

    Lysates/proteins at 1 µg per lane.

文献

This product has been referenced in:
  • Cheng D  et al. Biogenesis and cytotoxicity of APOL1 renal risk variant proteins in hepatocytes and hepatoma cells. J Lipid Res 56:1583-93 (2015). Read more (PubMed: 26089538) »
  • Arroteia KF  et al. Albumin is synthesized in epididymis and aggregates in a high molecular mass glycoprotein complex involved in sperm-egg fertilization. PLoS One 9:e103566 (2014). WB, IP ; Mouse . Read more (PubMed: 25084016) »

See all 3 Publications for this product

客户评价及客户问答

Thank you for contacting us.
As we haven't tested the cross-reactivity for this antibodies, it is very much likely that they will react with other albumins.
Therefore, I would suggest to use milk as a blocking agent (0.5% Skim milk/PBS for 1 h...

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Further to our telephone conversation I have been in touch with the lab with regards the source of this immunogen for use as a positive control. Unfortunately they were unsure where the mouse serum albumin was sourced from. I can only recommend tha...

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Thank you for your enquiry. I have been in touch with the lab and I can confirm that rabbit polyclonal to Mouse Serum Albumin (ab34807) was tested by ELISA by coating each well with 1ug mouse serum albumin. The antibody was then added to the wells i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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