概述

  • 产品名称小鼠SCF ELISA试剂盒
    参阅全部 SCF 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 5 pg/ml
  • 范围
    2.74 pg/ml - 2000 pg/ml
  • 复检

    96 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 98.64 85% - 106%
    Serum 95.86 85% - 105%
    Plasma 94.56 84% - 104%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse, Rat
  • 产品概述

    Abcam’s SCF Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse SCF in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse SCF coated on a 96-well plate. Standards and samples are pipetted into the wells and SCF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse SCF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SCF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of mast cells and is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also mediates cell to cell adhesion and acts synergistically with other cytokines, probably interleukins. A soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain.
  • 别名
    • C kit ligand
    • Ckit ligand
    • Kit ligand
    • Kitl
    • KITLG
    • KL 1
    • KL1
    • Mast cell growth factor
    • MGF
    • SF
    • SHEP7
    • Steel factor
    • Stem cell factor
    • Stem cell factor precursor
    see all

应用

Our Abpromise guarantee covers the use of ab100740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse SCF ELISA Kit 图像

  • SCF measured in various fluids showing quantity (pg) per mL of tested sample

  • Representative standard curve using ab100740

  • Representative standard curve using ab100740

实验方案

Mouse SCF ELISA Kit (ab100740)参考文献

This product has been referenced in:
  • Lu H  et al. Prevention of adverse cardiac remodeling to volume overload in female rats is the result of an estrogen-altered mast cell phenotype. Am J Physiol Heart Circ Physiol 302:H811-7 (2012). Rat . Read more (PubMed: 22160000) »
  • Li J  et al. Stem cell factor is responsible for the rapid response in mature mast cell density in the acutely stressed heart. J Mol Cell Cardiol 53:469-74 (2012). Read more (PubMed: 22850284) »

See all 2 Publications for this product

Product Wall

Thank you for your inquiry.

Unfortunately, we do not have any cross reactivity data for testing rat samples using the mouse stem cell factor ELISA kit (ab100740). Based on online literature though, mouse SCF has ˜93% homology with rat SCF so...

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Thank you for your call today and for letting us know about the trouble with this kit.
As we discussed, I'm sending a free of charge kit which should arrive around Tuesday of next week on the order ***. I have requested that the components are che...

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Thank you for contacting us.

First, I want to apologize to you for the delay in gathering this information. I hope this did not cause you any inconveniences.

In regards to using rat tissues in our SCF Mouse ELISA kit, there is no ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"