小鼠RANK ELISA试剂盒(CD265) (ab119606)

概述

  • 产品名称小鼠RANK ELISA试剂盒(CD265)
    参阅全部 RANK 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 2 pg/ml
  • 范围
    62.5 pg/ml - 4000 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s Mouse RANK (CD265) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse RANK (CD265) in cell culture supernatants and serum. 

    A RANK (CD265) specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for RANK is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse RANK amount of sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells.
  • 组织特异性Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.
  • 疾病相关Defects in TNFRSF11A are the cause of familial expansile osteolysis (FEO) [MIM:174810]. FEO is a rare autosomal dominant bone disorder characterized by focal areas of increased bone remodeling. The osteolytic lesions develop usually in the long bones during early adulthood. FEO is often associated with early onset deafness and loss of dentition.
    Defects in TNFRSF11A are a cause of Paget disease of bone type 2 (PDB2) [MIM:602080]; also known as familial Paget disease of bone. PDB2 is a bone-remodeling disorder with clinical similarities to FEO. Unlike FEO, however, affected individuals have involvement of the axial skeleton with lesions in the spine, pelvis and skull.
    Defects in TNFRSF11A are the cause of osteopetrosis autosomal recessive type 7 (OPTB7) [MIM:612301]; also called osteoclast-poor osteopetrosis with hypogammaglobulinemia. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. OPTB7 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. OPTB7 is associated with hypogammaglobulinemia.
  • 序列相似性Contains 4 TNFR-Cys repeats.
  • 细胞定位Membrane.
  • Information by UniProt
  • 别名
    • CD265
    • FEO
    • LOH18CR1
    • Loss of heterozygosity 18 chromosomal region 1
    • ODFR
    • OFE
    • OPTB7
    • Osteoclast differentiation factor receptor
    • OSTS
    • PDB2
    • Receptor activator of NF-KB
    • Receptor activator of nuclear factor kappa B
    • TNFRSF11A
    • TNR11_HUMAN
    • TRANCER
    • Tumor necrosis factor receptor superfamily member 11A
    • Tumor necrosis factor receptor superfamily member 11a activator of NFKB
    • Tumor necrosis factor receptor superfamily member 11a NFKB activator
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse RANK ELISA Kit (CD265) 图像

  • Representative standard curve using ab119606

实验方案

Mouse RANK ELISA Kit (CD265) (ab119606)参考文献

ab119606 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email.

Yes, we do have OPG kits available. The catalogue number is ab100617 and ab100733, one is specific to human and other one to mouse.

http://www.abcam.com/Osteoprotegerin-Human-ELISA-Kit-1-x-96-Well-Plate-ab100...

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Thank you for contacting us.

Could you please provide the species you are interested in? We have mouse RANKL ELISA kit to use for serum as well as plasma.

http://www.abcam.com/RANKL-mouse-ELISA-Kit-1-x-96-Well-Plate-ab100749.html
...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"