小鼠RAGE ELISA试剂盒(ab100738)

概述

  • 产品名称
    小鼠RAGE ELISA试剂盒
    参阅全部 RAGE 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 15 pg/ml
  • 范围
    13.72 pg/ml - 10000 pg/ml
  • 复检

    87 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 75.89 67% - 82%
    Serum 102.9 91% - 110%
    Plasma 82.69 71% - 92%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s RAGE Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse RAGE in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse RAGE coated on a 96-well plate. Standards and samples are pipetted into the wells and RAGE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse RAGE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RAGE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab100738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Standard curve of mouse RAGE with background signal subtracted (duplicates; +/- SD).

  • Mouse RAGE measured in in mouse tissue lysates (in RIPA buffer tested in the range of 0.1ug to 0.1 mg protein, quantity of RAGE shown as per mg of extracted protein ), duplicates +/- SD.

  • Mouse RAGE measured in biological fluids (dilution range neat - 1/10; duplicates +/- SD).

  • Representative standard curve using ab100738

实验方案

文献

ab100738 has not yet been referenced specifically in any publications.

客户评价及客户问答

Tha lab sent their data for the two lots that gave you low standard OD compared to the June assay.

October


Standard (pg/ml) OD
10,000 2.832
3,333 1.512
1,111 0.732
370.4 0.351
123.5 0.201
41.15 0....

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Thank you for contacting us.

The recommended dilution for testing normal mouse serum and plasma with this kit is 2-fold.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or in...

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Thank you for contacting us.  This ELISA kit has not been validated for use on tissue samples, but may be suitable for that purpose.  Theoretically, any low-salt lysis buffer that meets the following criteria should be compatible with this kit: ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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