概述

  • 产品名称小鼠proMMP9 ELISA试剂盒
    参阅全部 MMP9 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 50 pg/ml
  • 范围
    74.07 pg/ml - 18000 pg/ml
  • 复检

    100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 109.7 96% - 118%
    Serum 114.4 97% - 132%
    Plasma 112 101% - 121%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    proMMP9 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab100732) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse proMMP9 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for mouse proMMP9 coated on a 96-well plate. Standards and samples are pipetted into the wells and proMMP9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse proMMP9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of proMMP9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
    -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • 组织特异性Produced by normal alveolar macrophages and granulocytes.
  • 疾病相关Intervertebral disc disease
    Metaphyseal anadysplasia 2
  • 序列相似性Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin repeats.
  • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    N- and O-glycosylated.
  • 细胞定位Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 别名
    • 82 kDa matrix metalloproteinase-9
    • 92 kDa gelatinase
    • 92 kDa type IV collagenase
    • CLG 4B
    • CLG4B
    • Collagenase Type 4 beta
    • Collagenase type IV 92 KD
    • EC 3.4.24.35
    • Gelatinase 92 KD
    • Gelatinase B
    • Gelatinase beta
    • GelatinaseB
    • GELB
    • Macrophage gelatinase
    • MANDP2
    • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
    • Matrix Metalloproteinase 9
    • MMP 9
    • MMP-9
    • MMP9
    • MMP9_HUMAN
    • Type V collagenase
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse proMMP9 ELISA Kit 图像

  • Representative standard curve using ab100732

实验方案

Mouse proMMP9 ELISA Kit (ab100732)参考文献

ab100732 has not yet been referenced specifically in any publications.

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