概述

  • 产品名称小鼠Prolactin ELISA试剂盒
    参阅全部 Prolactin 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 30 pg/ml
  • 范围
    27.43 pg/ml - 20000 pg/ml
  • 复检

    81 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 76.85 68% - 87%
    Serum 82.47 73% - 94%
    Plasma 76.78 67% - 85%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s Prolactin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Prolactin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse Prolactin coated on a 96-well plate. Standards and samples are pipetted into the wells and Prolactin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Prolactin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Prolactin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands. Prolactin acts primarily on the mammary gland by promoting lactation.
  • 细胞定位Secreted
  • 别名
    • AV290867
    • Lactogenic Hormone
    • Luteotropic Hormone
    • Mammotropin
    • OTTMUSP00000000651
    • PRL
    • Prl1a1
    • Prol
    • Prolactin precursor
    • RATPRLSD1
    • RNPROL
    • RP23-351I6.2
    • RP3-404K8.1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse Prolactin ELISA Kit 图像

  • Prolactin measured in mouse biological fluids showing quantity (pg) per mL of tested sample. Samples diluted 10-270 fold.

  • Representative standard curve using ab100736

  • Representative standard curve using ab100736

实验方案

Mouse Prolactin ELISA Kit (ab100736)参考文献

This product has been referenced in:
  • Golub Y  et al. Effects of In utero environment and maternal behavior on neuroendocrine and behavioral alterations in a mouse model of prenatal trauma. Dev Neurobiol 76:1254-1265 (2016). Read more (PubMed: 26899175) »
  • O'Hara L  et al. Pituitary androgen receptor signalling regulates prolactin but not gonadotrophins in the male mouse. PLoS One 10:e0121657 (2015). Mouse . Read more (PubMed: 25799562) »

See all 4 Publications for this product

Product Wall



Wir empfehlen eine 5- 100 fache Verdünnung für Serumproben. Da der Level an Zielprotein sehr variieren kann zwischen verschiedenen Proben muss der optimale Verdünnungsfaktor experimentell vom Benutzer herausgefunden werden. Wir...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Thank you for contacting us. I apologize for any inconvenience.

I can confirm that the spiky bottomed vial is Item-F (Biotinylated anti mouse antibody) and the round bottomed vials Item C are recombinant prolacticn.

Please let me kno...

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I am sorry you received a kit which contained a contaminated buffer and for any trouble this has caused. As requested, I have issued a free of charge replacement for one kit of ab100736.

To check the status of the order please contact our Cu...

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Thanks so much for all your troubleshooting efforts and for sending along the pictures. I agree that whatever that is does not look like precipitation. I would be happy to send you a replacement of ab100736.

If you could, please send me the P...

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Unless there is some discoloration I don’t think you have much to worry about especially if the standard curve looks fine as it is likely just precipitation. Do you have a photo you could send me?

As the diluent does contain proteins a...

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Thank you for your inquiry. Please see my answers to your questions below: 1. I can confirm that there are special Serum collection tubes commercially available from many vendors. But serum can also easily be extracted by your customer by hand....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"