小鼠PPBP ELISA试剂盒(CXCL7) (ab100742)

概述

  • 产品名称小鼠PPBP ELISA试剂盒(CXCL7)
    参阅全部 CXCL7 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 14 pg/ml
  • 范围
    10.97 pg/ml - 8000 pg/ml
  • 复检

    100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 123.5 108% - 133%
    Serum 118.3 100% - 135%
    Plasma 97.75 75% - 110%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s PPBP Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse PPBP in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse PPBP coated on a 96- well plate. Standards and samples are pipetted into the wells and PPBP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse PPBP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PPBP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性CXCL7 is a platelet-derived growth factor that belongs to the CXC chemokine family. It is a potent chemoattractant and activator of neutrophils and has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.
  • 别名
    • B TG1
    • Beta TG
    • Beta thromboglobulin
    • C X C motif chemokine 7
    • Connective tissue activating peptide III
    • CTAP III
    • CTAP3
    • CTAPIII
    • LA PF4
    • LDGF
    • Leukocyte derived growth factor
    • Low affinity platelet factor IV
    • Macrophage derived growth factor
    • MDGF
    • NAP 2
    • Neutrophil activating peptide 2
    • PBP
    • Platelet basic protein
    • Ppbp
    • pro platelet basic protein (chemokine (C X C motif) ligand 7)
    • SCYB7
    • Small inducible cytokine B7
    • Small inducible cytokine subfamily B, member 7
    • TC1
    • TC2
    • TCK 1
    • TGB
    • TGB1
    • THBGB
    • THBGB1
    • Thrombocidin 1
    • Thrombocidin 2
    • Thymus Chemokine 1
    see all

应用

Our Abpromise guarantee covers the use of ab100742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse PPBP ELISA Kit (CXCL7) 图像

  • Representative Standard Curve useing ab100742

实验方案

Mouse PPBP ELISA Kit (CXCL7) (ab100742)参考文献

ab100742 has not yet been referenced specifically in any publications.

Product Wall


The lab let me know that sodium citrate is also an acceptable anti-coagulant.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"