概述

  • 产品名称小鼠PF4 ELISA试剂盒(CXCL4)
    参阅全部 PF4 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 6 pg/ml
  • 范围
    4.12 pg/ml - 3000 pg/ml
  • 复检

    100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 73.99 67% - 83%
    Serum 118.2 104% - 127%
    Plasma 109.3 92% - 129%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s PF4 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse PF4 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse PF4 coated on a 96-well plate. Standards and samples are pipetted into the wells and PF4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse PF4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PF4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
  • 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 翻译后修饰Binds non-covalently to a proteoglycan molecule.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • C-X-C motif chemokine 4
    • Chemokine (C X C motif) ligand 4
    • Chemokine (CXC motif) ligand 4
    • CXCL 4
    • CXCL4
    • Iroplact
    • MGC138298
    • Oncostatin A
    • Oncostatin-A
    • OncostatinA
    • PF 4
    • PF-4
    • PF4
    • Platelet factor 4
    • PLF4_HUMAN
    • SCYB 4
    • SCYB4
    • short form
    • Small inducible cytokine subfamily member 4
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse PF4 ELISA Kit (CXCL4) 图像

  • PF4 measured in cell supernatanses showing quantity (pg) per mL tested sample

  • PF4 measured in biofluids showing quantity (microgram) per mL of tested sample

  • Representative standard curve using ab100735

  • Representative standard curve using ab100735

实验方案

Mouse PF4 ELISA Kit (CXCL4) (ab100735)参考文献

This product has been referenced in:
  • Petzold T  et al. ß1 integrin-mediated signals are required for platelet granule secretion and hemostasis in mouse. Blood 122:2723-31 (2013). Mouse . Read more (PubMed: 24004668) »

See 1 Publication for this product

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The lab let me know that sodium citrate is also an acceptable anti-coagulant.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"