小鼠Osteopontin ELISA试剂盒(ab100734)

概述

  • 产品名称小鼠Osteopontin ELISA试剂盒
    参阅全部 Osteopontin 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 4 pg/ml
  • 范围
    2.74 pg/ml - 2000 pg/ml
  • 复检

    100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 85.46 78% - 105%
    Serum 115.6 103% - 122%
    Plasma 110.4 87% - 130%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s Osteopontin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Osteopontin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Osteopontin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteopontin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Osteopontin antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteopontin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
    Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • 组织特异性Bone. Found in plasma.
  • 序列相似性Belongs to the osteopontin family.
  • 翻译后修饰Extensively phosphorylated on clustered serine residues.
    N- and O-glycosylated.
    Phosphorylation sites are present in the extracelllular medium.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • BNSP
    • Bone sialoprotein 1
    • BSP I
    • BSPI
    • Early T lymphocyte activation 1
    • ETA 1
    • ETA1
    • MGC110940
    • Nephropontin
    • OPN
    • Osteopontin
    • osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein
    • OSTP_HUMAN
    • PSEC0156
    • Secreted phosphoprotein 1
    • secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
    • SPP 1
    • SPP-1
    • SPP1
    • SPP1/CALPHA1 fusion
    • Urinary stone protein
    • Uropontin
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse Osteopontin ELISA Kit 图像

  • Representative standard curve using ab100734

实验方案

Mouse Osteopontin ELISA Kit (ab100734)参考文献

This product has been referenced in:
  • Wong TM  et al. Respiratory syncytial virus (RSV) infection in elderly mice results in altered antiviral gene expression and enhanced pathology. PLoS One 9:e88764 (2014). Read more (PubMed: 24558422) »
  • Fang X  et al. Ameliorated ConA-induced hepatitis in the absence of PKC-theta. PLoS One 7:e31174 (2012). Mouse . Read more (PubMed: 22347449) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.

The mouse OPN kit does have a very low level cross-reactivity with human OPN. We’ve measure it to around 2-3% cross-reactivity. This is low enough I think you could measure it in your sample, but you might ne...

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Abreview for Osteopontin Mouse ELISA Kit

Good Good 4/5 (Ease of Use)
Abreviews

Sample: Mouse (Lavage fluid)
Specification: Lavage fluid
Amount of Sample: Dilutions of pooled lavage µg
Type of Kit Used: ELISA
Positive control: recombinant Osteopontin spiked in diluent
Did you use the Abcam Kit protocol?: Yes
Username

Abcam user community

Verified customer

提交于 Mar 18 2013

Thank youfor your inquiry.

The quote for ab8448 has been send in a separate email.

I am happy to confirm that we offer ELISA kits for Osteopontin:

ab100618 (Osteopontin Human ELISA Kit)

http://www.abcam.com/index...

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Thank you for contacting us.

This kit has not been optimised for use with tissue lysates but should be suitable for that purpose.

For the sample preparation, please note that there should not be more than 0.1% SDS present in the bu...

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Thank you for contacting us.  This kit has not been optimized for use with tissue lysates but should be suitable for that purpose.  A RIPA buffer should be fine as long as it meets the lysis buffer specifications below.  Please note that there should n...

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Abreview for Osteopontin Mouse ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Mouse Tissue sections (Lung homogenate)
Specification: Lung homogenate
Amount of Sample: 10 µg
Type of Kit Used: ELISA
Positive control: Recombinant mouse OPN of known concentration.
Did you use the Abcam Kit protocol?: Yes
Username

Abcam user community

Verified customer

提交于 May 13 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"