小鼠Myoglobin ELISA试剂盒(ab157722)
Key features and details
- Sensitivity: 4.48 ng/ml
- Range: 9.375 ng/ml - 300 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠Myoglobin ELISA试剂盒
参阅全部 Myoglobin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 10% -
样品类型
Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
4.48 ng/ml -
范围
9.375 ng/ml - 300 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum > 85 % - % -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s Myoglobin Mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Myoglobin in mouse samples.
In this assay the Myoglobin present in samples reacts with the anti-Myoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Myoglobin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Myoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Myoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Myoglobin in the test sample. The quantity of Myoglobin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Get better reproducibility in only 90 minutes with Mouse Myoglobin ELISA Kit (ab210965) from our SimpleStep ELISA® range.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 100X HRP-conjugated anti-mouse Myoglobin antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Mouse Myoglobin Calibrator (Lyophilized) 1 vial Mouse Myoglobin ELISA Microplate 1 unit Stop Solution 1 x 12ml -
研究领域
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功能
Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. -
序列相似性
Belongs to the globin family. - Information by UniProt
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别名
- MB
- MGC13548
- MYG_HUMAN
- Myoglobin
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数据库链接
- Entrez Gene: 17189 Mouse
- SwissProt: P04247 Mouse
- Unigene: 404074 Mouse
相关产品
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SimpleStep ELISA kits
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab157722 被引用在 1 文献中.
- Warfel JD et al. A low fat diet ameliorates pathology but retains beneficial effects associated with CPT1b knockout in skeletal muscle. PLoS One 12:e0188850 (2017). ELISA ; Mouse . PubMed: 29240830