小鼠单克隆抗体[Mp 4H2]抗人IgG heavy chain (ab9243)

概述

  • 产品名称
    小鼠单克隆抗体[Mp 4H2]抗人IgG heavy chain
    参阅全部 IgG 二抗
  • 宿主
    Mouse
  • 靶标种属
    Human
  • 特异性
    This antibody is specific for the gamma heavy chain of human IgG
  • 经测试应用
    适用于: ELISA, IHC-Fr, WBmore details
  • 免疫原

    Purified intact human IgG

  • 阳性对照
    normal human serum Ig Fraction

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Constituent: PBS
  • Concentration information loading...
  • 纯度
    Ascites
  • 克隆
    单克隆
  • 克隆编号
    Mp 4H2
  • 同种型
    IgG1
  • 轻链类型
    kappa
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent dilution.
WB Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 25, 50 -150 kDa. The antibody recognizes the heavy chains of human IgG. The size of the band observed depends on the sample preparation and degree of fragmentation the Ig molecule has undergone. Under reduced conditions in WB heavy chains at 50 kD. Intact (native) IgG is 150 kDa. Therefore either 50 kDa or the entire IgG at 150kDa will be observed, depending on how much the molecule is fragmented (you may see both).

文献

This product has been referenced in:
  • Gieffers C  et al. APG350 induces superior clustering of TRAIL receptors and shows therapeutic antitumor efficacy independent of cross-linking via Fc? receptors. Mol Cancer Ther 12:2735-47 (2013). Read more (PubMed: 24101228) »

See 1 Publication for this product

客户评价及客户问答

Thank you for your inquiry.

Please find the CoC in the attachment.

I hope this is helpful. Please do not hesitate to contact us again, should you have any further question.

Thank you for contacting Abcam.

We have not tested any of those antibodies against monkey IgG, therefore I cannot say whether they will cross react or not. Due to this, you can take advantage of our testing discount program if you want, pleas...

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Ab9243 has been used to identify human IgG in ELISA. The antibody's originator has not seen any cross-reaction to other isotypes. If you have any more questions please contact us again.

This antibody is specific for the human IgG heavy chain, but its epitope has not been mapped further. However, this antibody has been used to test chimeric antibodies where the Fab region is human. As ab9243 works well for this testing, one can assume ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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