小鼠单克隆抗体Mp 4H2 Anti-Human IgG heavy chain (ab9243)

概述

  • 产品名称小鼠单克隆抗体Mp 4H2 Anti-Human IgG heavy chain
    参阅全部 IgG 二抗
  • 靶标种属Human
  • 特异性This antibody is specific for the gamma heavy chain of human IgG
  • 经测试应用适用于: ELISA, IHC-Fr, WBmore details
  • 免疫原

    Purified intact human IgG

  • 阳性对照normal human serum Ig Fraction

性能

应用

Our Abpromise guarantee covers the use of ab9243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent dilution.
WB Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 25, 50 -150 kDa. The antibody recognizes the heavy chains of human IgG. The size of the band observed depends on the sample preparation and degree of fragmentation the Ig molecule has undergone. Under reduced conditions in WB heavy chains at 50 kD. Intact (native) IgG is 150 kDa. Therefore either 50 kDa or the entire IgG at 150kDa will be observed, depending on how much the molecule is fragmented (you may see both).

Mouse monoclonal Mp 4H2 Anti-Human IgG heavy chain (ab9243)参考文献

This product has been referenced in:
  • Gieffers C  et al. APG350 induces superior clustering of TRAIL receptors and shows therapeutic antitumor efficacy independent of cross-linking via Fc? receptors. Mol Cancer Ther 12:2735-47 (2013). Read more (PubMed: 24101228) »

See 1 Publication for this product

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We have not tested any of those antibodies against monkey IgG, therefore I cannot say whether they will cross react or not. Due to this, you can take advantage of our testing discount program if you want, pleas...

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Ab9243 has been used to identify human IgG in ELISA. The antibody's originator has not seen any cross-reaction to other isotypes. If you have any more questions please contact us again.

This antibody is specific for the human IgG heavy chain, but its epitope has not been mapped further. However, this antibody has been used to test chimeric antibodies where the Fab region is human. As ab9243 works well for this testing, one can assume ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"