小鼠单克隆抗体HP-6017 Anti-Human IgG Fc (FITC) (ab49867)

概述

  • 产品名称小鼠单克隆抗体HP-6017 Anti-Human IgG Fc (FITC)
    参阅全部 IgG 二抗
  • 靶标种属Human
  • 特异性This antibody reacts specifically with IgG (Fc fragment), all subclasses. The antibody does not react with IgA, IgM, purified light chains or the Fab fragment of IgG. This clone has been evaluated for specificity using a wide range of immunological techniques in the IUIS/WHO collaborative study and has been adopted by IUIS/WHO study as a standard reagent.
  • 经测试应用适用于: Dot blotmore details
  • 免疫原

    Human IgG coupled to polyaminostyrene beads.

  • 偶联物FITC. Ex: 493nm, Em: 528nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: 1% BSA (inactivated), 0.01M PBS, pH 8.0
  • Concentration information loading...
  • 纯度IgG fraction
  • 共轭说明The immunoglobulin fraction of the ascites fluid was conjugated to fluorescein isothiocyanate (FITC) and then further purified to remove unconjugated FITC. F/P Molar Ratio: 3 to 5.
  • 克隆单克隆
  • 克隆编号HP-6017
  • 同种型IgG2a
  • 常规说明

    Although the antibody site is located in the terminal end of the human IgG molecule (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor (autoantibodies directed to Fc), passage through placental membrane and staphylococcus protein A binding. A certain population of the lymphocytes posseses the "Fc receptor", therefore specific immunoreagents targeted only for the Fc fragment should prove useful.

    F-DIBA (Fluorescent Dot Immunobinding Assay): Minimum dilution of 1/16 for the conjugate on a 4-8µg dot of Human IgG (Fc fragment). F-IFMA (Particle Immunofluorescent Assay): Minimum dilution of 1/16 for the conjugate on 50µl suspension of Human IgG Agarose coated with approximately 20mg Human IgG.

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab49867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot 1/16.

Mouse monoclonal HP-6017 Anti-Human IgG Fc (FITC) (ab49867)参考文献

ab49867 has not yet been referenced specifically in any publications.

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