小鼠单克隆抗体2A 8F4 Anti-Rat IgG2a heavy chain (Phycoerythrin) (ab99669)

概述

  • 产品名称小鼠单克隆抗体2A 8F4 Anti-Rat IgG2a heavy chain (Phycoerythrin)
    参阅全部 IgG2a 二抗
  • 描述
    小鼠单克隆抗体[2A 8F4]二抗to大鼠IgG2a - heavy chain (PE)
  • 靶标种属Rat
  • 特异性ab99669 reacts with the γ2a heavy chain (Fc) of Rat IgG2a.
  • 经测试应用适用于: ELISA, ICC/IF, Flow Cytmore details
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm

性能

应用

Our Abpromise guarantee covers the use of ab99669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
Flow Cyt Use 0.1µg for 106 cells.

Mouse monoclonal 2A 8F4 Anti-Rat IgG2a heavy chain (Phycoerythrin) 图像

  • BALB/c splenocytes were incubated with Rat IgG1 isotype control, Rat anti-Mouse CD22 (2D6, Rat IgG1), Rat anti-Mouse CD8 (53-6.7, Rat IgG2a), Rat anti-Mouse CD45 (LCA) (I3/2.3, Rat IgG2b), Rat anti-Mouse CD24 (30-F1, Rat IgG2c), and Rat anti-Mouse CD45RC (GL24, Rat IgM). After washing, the cells were stained with ab99699 at 0.02µg/106 cells. Small lymphocytes were gated and analyzed.
  • Top: (Negative control) ab99669 being used against BALB/c spleenocytes at 0.1 µg/106 cells.
    Bottom: ab99669 staining BALB/c spleenocytes incubated with rat anti-mouse CD8 (IgG2a isotype) at 0.1 µg/106 cells.

Mouse monoclonal 2A 8F4 Anti-Rat IgG2a heavy chain (Phycoerythrin) (ab99669)参考文献

ab99669 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"