小鼠MIP3 beta ELISA试剂盒(CCL19) (ab100729)

概述

  • 产品名称小鼠MIP3 beta ELISA试剂盒(CCL19)
    参阅全部 Macrophage Inflammatory Protein 3 beta 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 5 pg/ml
  • 范围
    4.1 pg/ml - 1000 pg/ml
  • 复检

    100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 85.6 78% - 101%
    Serum 107.7 82% - 132%
    Plasma 119.5 83% - 140%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s MIP3 beta Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP-3- beta in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse MIP3 beta coated on a 96- well plate. Standards and samples are pipetted into the wells and MIP3 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP3 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes.
  • 组织特异性Expressed at high levels in the lymph nodes, thymus and appendix. Intermediate levels seen in colon and trachea, while low levels found in spleen, small intestine, lung, kidney and stomach.
  • 序列相似性Belongs to the intercrine beta (chemokine CC) family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Beta chemokine exodus 3
    • Beta-chemokine exodus-3
    • C C chemokine ligand 19
    • C-C motif chemokine 19
    • CC chemokine ligand 19
    • CCL 19
    • CCL19
    • CCL19_HUMAN
    • Chemokine (C C motif) ligand 19
    • Chemokine (CC motif) ligand 19
    • Chemokine C C Motif Ligand 19
    • Chemokine CC Motif Ligand 19
    • CK beta 11
    • CK beta-11
    • CKb 11
    • CKb11
    • EBI 1 ligand chemokine
    • EBI1 ligand chemokine
    • ELC
    • Epstein-Barr virus-induced molecule 1 ligand chemokine
    • Exodus 3
    • Exodus3
    • Macrophage inflammatory protein 3 beta
    • MGC34433
    • MIP 3 beta
    • MIP 3B
    • MIP-3-beta
    • MIP-3b
    • MIP3 beta
    • MIP3B
    • OTTHUMP00000000531
    • SCYA 19
    • SCYA19
    • Small inducible cytokine A19
    • Small inducible cytokine subfamily A (Cys Cys) member 1
    • Small-inducible cytokine A19
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse MIP3 beta ELISA Kit (CCL19) 图像

  • Representative standard curve using ab100729

实验方案

Mouse MIP3 beta ELISA Kit (CCL19) (ab100729)参考文献

ab100729 has not yet been referenced specifically in any publications.

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