小鼠MIP3 beta ELISA试剂盒(CCL19) (ab100729)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
-
产品名称
小鼠MIP3 beta ELISA试剂盒(CCL19)
参阅全部 MIP-3 beta/CCL19 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 5 pg/ml -
范围
4.1 pg/ml - 1000 pg/ml -
回收率
100 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 85.6 78% - 101% Serum 107.7 82% - 132% Plasma 119.5 83% - 140% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s MIP3 beta Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP-3- beta in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse MIP3 beta coated on a 96- well plate. Standards and samples are pipetted into the wells and MIP3 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP3 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 260X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated anti-Mouse MIP3 beta 2 vials MIP3 beta Microplate (12 x 8 wells) 1 unit Recombinant Mouse MIP3 beta Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
-
功能
May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. -
组织特异性
Expressed at high levels in the lymph nodes, thymus and appendix. Intermediate levels seen in colon and trachea, while low levels found in spleen, small intestine, lung, kidney and stomach. -
序列相似性
Belongs to the intercrine beta (chemokine CC) family. -
细胞定位
Secreted. - Information by UniProt
-
别名
- Beta chemokine exodus 3
- Beta-chemokine exodus-3
- C C chemokine ligand 19
see all -
数据库链接
- Entrez Gene: 100039053 Mouse
- Entrez Gene: 24047 Mouse
- SwissProt: O70460 Mouse
- Unigene: 426373 Mouse
- Unigene: 441169 Mouse
- Unigene: 459263 Mouse
- Unigene: 6660 Mouse
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab100729 被引用在 2 文献中.
- Iida Y et al. Local injection of CCL19-expressing mesenchymal stem cells augments the therapeutic efficacy of anti-PD-L1 antibody by promoting infiltration of immune cells. J Immunother Cancer 8:N/A (2020). PubMed: 32675195
- Jenkins RW et al. Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids. Cancer Discov 8:196-215 (2018). PubMed: 29101162