为了使您在Abcam官网的浏览体验更顺畅，请使用最新版本的浏览器比如 Google Chrome
|Cell culture supernatant||80.52||72% - 91%|
|Serum||83.74||74% - 93%|
|Plasma||108.2||84% - 143%|
Abcam’s MIP3 alpha Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP3 alpha in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse MIP3 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP3 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP3 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|组件||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|400X HRP-Streptavidin Concentrate||1 unit|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-mouse MIP3 alpha||2 vials|
|MIP3 alpha Microplate (12 x 8 wells)||1 unit|
|Recombinant Mouse MIP3 alpha Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100728 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"