小鼠MIP3 alpha ELISA试剂盒(CCL20) (ab100728)
概述
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产品名称小鼠MIP3 alpha ELISA试剂盒(CCL20)
参阅全部 Macrophage Inflammatory Protein 3 alpha 试剂盒 -
检测方法Colorimetric
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样品类型Cell culture supernatant, Serum, Plasma
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检测类型Sandwich (quantitative)
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灵敏度< 3 pg/ml
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范围2.06 pg/ml - 1500 pg/ml
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复检
90 %
特定样本复检结果 样品类型 平均% 范围 Cell culture supernatant 80.52 72% - 91% Serum 83.74 74% - 93% Plasma 108.2 84% - 143% -
实验步骤Multiple steps standard assay
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种属反应性与反应: Mouse
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产品概述
Abcam’s MIP3 alpha Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP3 alpha in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse MIP3 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP3 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP3 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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经测试应用适用于: Sandwich ELISAmore details
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平台Microplate
性能
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存放说明Store at -20°C. Please refer to protocols.
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组件 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 unit 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-mouse MIP3 alpha 2 vials MIP3 alpha Microplate (12 x 8 wells) 1 unit Recombinant Mouse MIP3 alpha Standard 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213.
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组织特异性Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.
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序列相似性Belongs to the intercrine beta (chemokine CC) family.
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翻译后修饰C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
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细胞定位Secreted.
- Information by UniProt
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别名
- Beta-chemokine exodus-1
- C C motif chemokine ligand 20
- C-C motif chemokine 20
see all -
数据库链接
- Entrez Gene: 20297 Mouse
- SwissProt: O89093 Mouse
应用
Our Abpromise guarantee covers the use of ab100728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Mouse MIP3 alpha ELISA Kit (CCL20) 图像
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Typical standard curveRepresentative standard curve using ab100728
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Typical standard curveRepresentative standard curve using ab100728
实验方案
Mouse MIP3 alpha ELISA Kit (CCL20) (ab100728)参考文献
ab100728 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"