小鼠MIP2 ELISA试剂盒(CXCL2) (ab100727)

概述

  • 产品名称小鼠MIP2 ELISA试剂盒(CXCL2)
    参阅全部 CXCL2 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 8 pg/ml
  • 范围
    4.1 pg/ml - 1000 pg/ml
  • 复检

    90 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 94.58 86% - 104%
    Serum 88.58 83% - 99%
    Plasma 87.94 82% - 98%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s MIP2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP2 in serum, plasma and cell culture supernatants. (Mouse MIP2 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

    This assay employs an antibody specific for mouse MIP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
  • 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 翻译后修饰The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • C-X-C motif chemokine 2
    • Chemokine (C-X-C motif) ligand 2
    • CINC-2a
    • Cxcl2
    • CXCL2_HUMAN
    • Gro beta
    • Gro-beta
    • GRO-beta(5-73)
    • GRO-beta-T
    • GRO2
    • GRO2 oncogene
    • GROb
    • Growth-regulated protein beta
    • Hematopoietic synergistic factor
    • HSF
    • Macrophage inflammatory protein 2-alpha
    • Melanoma growth stimulatory activity beta
    • MGSA beta
    • MGSA-b
    • MIP 2
    • MIP 2a
    • MIP2
    • MIP2-alpha
    • MIP2A
    • SB-251353
    • SCYB2
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse MIP2 ELISA Kit (CXCL2) 图像

  • Representative standard curve using ab100727

  • Representative standard curve using ab100727

实验方案

Mouse MIP2 ELISA Kit (CXCL2) (ab100727)参考文献

ab100727 has not yet been referenced specifically in any publications.

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