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|Cell culture supernatant||94.58||86% - 104%|
|Plasma||87.94||82% - 98%|
Abcam’s MIP2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP2 in plasma and cell culture supernatants. (Mouse MIP2 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for mouse MIP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Mouse MIP2 in normal serum with ab100727, only in serum spiked with Mouse MIP2.
|组件||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|700X HRP-Streptavidin Concentrate||1 x 200µl|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-mouse MIP2 (lyophilized)||2 vials|
|MIP2 Microplate (12 x 8 wells)||1 unit|
|Recombinant Mouse MIP2 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100727 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"