小鼠MIP2 ELISA试剂盒(CXCL2) (ab100727)
Key features and details
- Sensitivity: 8 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
-
产品名称
小鼠MIP2 ELISA试剂盒(CXCL2)
参阅全部 CXCL2 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 8 pg/ml -
范围
4.1 pg/ml - 1000 pg/ml -
回收率
90 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 94.58 86% - 104% Plasma 87.94 82% - 98% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s MIP2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP2 in plasma and cell culture supernatants. (Mouse MIP2 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for mouse MIP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Mouse MIP2 in normal serum with ab100727, only in serum spiked with Mouse MIP2.
-
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Mouse MIP2 2 vials MIP2 Microplate (12 x 8 wells) 1 unit Recombinant Mouse MIP2 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
-
功能
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻译后修饰
The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells. -
细胞定位
Secreted. - Information by UniProt
-
别名
- C-X-C motif chemokine 2
- Chemokine (C X C motif) ligand 2
- Chemokine, CXC motif, ligand 2
see all -
数据库链接
- Entrez Gene: 20310 Mouse
- SwissProt: P10889 Mouse
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab100727 被引用在 1 文献中.
- Hunt NJ et al. Quantum Dot Nanomedicine Formulations Dramatically Improve Pharmacological Properties and Alter Uptake Pathways of Metformin and Nicotinamide Mononucleotide in Aging Mice. ACS Nano 15:4710-4727 (2021). PubMed: 33626869