小鼠MIP1a ELISA试剂盒(CCL3) (ab100726)

概述

  • 产品名称
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 8 pg/ml
  • 范围
    4.1 pg/ml - 1000 pg/ml
  • 复检

    87 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 94.58 86% - 104%
    Serum 88.58 83% - 99%
    Plasma 87.94 82% - 98%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s MIP-1a Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP-1a in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for mouse MIP-1a coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP-1a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP-1a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP-1a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 功能
    Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
  • 序列相似性
    Belongs to the intercrine beta (chemokine CC) family.
  • 翻译后修饰
    N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • C C motif chemokine 3
    • CCL 3
    • CCL3
    • CCL3_HUMAN
    • Chemokine C C motif ligand 3
    • G0/G1 switch regulatory protein 19 1
    • G0/G1 switch regulatory protein 19-1
    • G0S19 1
    • G0S19 1 protein
    • Heparin binding chemotaxis protein
    • L2G25B
    • LD78 alpha
    • LD78-alpha(4-69)
    • LD78alpha
    • Macrophage inflammatory protein 1 alpha
    • Macrophage inflammatory protein 1-alpha
    • MIP 1 alpha
    • MIP 1A
    • MIP-1-alpha
    • MIP-1-alpha(4-69)
    • MIP1A
    • PAT 464.1
    • SCYA 3
    • SCYA3
    • SIS alpha
    • SIS beta
    • SIS-beta
    • Small inducible cytokine A3
    • small inducible cytokine A3 (homologous to mouse Mip-1a)
    • Small-inducible cytokine A3
    • Tonsillar lymphocyte LD78 alpha protein
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse MIP1a ELISA Kit (CCL3) 图像

  • Representative standard curve using ab100726

实验方案

Mouse MIP1a ELISA Kit (CCL3) (ab100726)参考文献

ab100726 has not yet been referenced specifically in any publications.

Product Wall

I purchased the ELISA sandwich kit ab100726 for quantification of CCL3 in mice samples. The kit is optimized, according to the vendor, for detection of CCL3 in cell supernatant, serum or plasma.
When I tried to use the kit to measure CCL3 concentration in spinal cord homogenates, I found that serial dilutions of these homogenates do not return a proporcional ammount of CCL3, and therefore I assume that some secondary products are causing an interference with the kit detection ability.
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提交于 Sep 13 2017

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