小鼠Milk Fat Globule 1 ELISA试剂盒(ab100724)
概述
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产品名称小鼠Milk Fat Globule 1 ELISA试剂盒
参阅全部 Milk Fat Globule 1 试剂盒 -
检测方法Colorimetric
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样品类型Cell culture supernatant, Serum, Plasma
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检测类型Sandwich (quantitative)
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灵敏度< 1 pg/ml
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范围0.823 pg/ml - 600 pg/ml
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回收率
98 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 110.1 102% - 120% Serum 96.1 90% - 108% Plasma 88.7 73% - 108% -
实验步骤Multiple steps standard assay
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种属反应性与反应: Mouse
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产品概述
Abcam’s Milk Fat Globule 1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of mouse Milk Fat Globule 1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse Milk Fat Globule 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Milk Fat Globule 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Milk Fat Globule 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Milk Fat Globule 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get better reproducibility in only 90 minutes with Mouse Milk Fat Globule 1 ELISA Kit (ab208981) from our SimpleStep ELISA® range.
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经测试应用适用于: Sandwich ELISAmore details
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平台Microplate
性能
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存放说明Store at -20°C. Please refer to protocols.
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组件 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated anti-mouse Milk Fat Globule 1 2 vials Milk Fat Globule 1 Microplate (12 x 8 wells) 1 unit Recombinant Mouse Milk Fat Globule 1 Standard 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.
Medin is the main constituent of aortic medial amyloid. -
组织特异性Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.
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序列相似性Contains 1 EGF-like domain.
Contains 2 F5/8 type C domains. -
翻译后修饰Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273.
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细胞定位Membrane. Secreted.
- Information by UniProt
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别名
- BA46
- Breast epithelial antigen BA46
- Contains Medin
see all -
数据库链接
- Entrez Gene: 17304 Mouse
- SwissProt: P21956 Mouse
相关产品
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SimpleStep ELISA kits
应用
Our Abpromise guarantee covers the use of ab100724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
图片
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Typical standard curveRepresentative standard curve using ab100724
实验方案
文献
ab100724 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"