小鼠Milk Fat Globule 1 ELISA试剂盒(ab100724)

概述

  • 产品名称小鼠Milk Fat Globule 1 ELISA试剂盒
    参阅全部 Milk Fat Globule 1 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 1 pg/ml
  • 范围
    0.823 pg/ml - 600 pg/ml
  • 复检

    98 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 110.1 102% - 120%
    Serum 96.1 90% - 108%
    Plasma 88.7 73% - 108%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s Milk Fat Globule 1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of mouse Milk Fat Globule 1 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse Milk Fat Globule 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Milk Fat Globule 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Milk Fat Globule 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Milk Fat Globule 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.
    Medin is the main constituent of aortic medial amyloid.
  • 组织特异性Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.
  • 序列相似性Contains 1 EGF-like domain.
    Contains 2 F5/8 type C domains.
  • 翻译后修饰Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273.
  • 细胞定位Membrane. Secreted.
  • Information by UniProt
  • 别名
    • BA46
    • Breast epithelial antigen BA46
    • Contains Medin
    • EDIL1
    • HMFG
    • hP47
    • HsT19888
    • Lactadherin
    • Lactadherin [Precursor]
    • Medin
    • MFG E8
    • MFG-E8
    • MFGE8
    • MFGM
    • MFGM_HUMAN
    • Milk fat globule EGF factor 8
    • Milk fat globule EGF factor 8 protein
    • Milk fat globule-EGF factor 8
    • O acetyl disialoganglioside synthase
    • OAcGD3S
    • SED1
    • SPAG10
    • Sperm associated antigen 10
    • Sperm surface protein hP47
    see all
  • 数据库链接

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应用

Our Abpromise guarantee covers the use of ab100724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse Milk Fat Globule 1 ELISA Kit 图像

  • Representative standard curve using ab100724

实验方案

Mouse Milk Fat Globule 1 ELISA Kit (ab100724)参考文献

ab100724 has not yet been referenced specifically in any publications.

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