小鼠MIG ELISA试剂盒(CXCL9) (ab100725)

概述

  • 产品名称
    小鼠MIG ELISA试剂盒(CXCL9)
    参阅全部 CXCL9 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 3 pg/ml
  • 范围
    2.741 pg/ml - 2000 pg/ml
  • 复检

    83 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 74.58 67% - 81%
    Serum 91.81 80% - 101%
    Plasma 85.55 77% - 93%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s MIG Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIG in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for mouse MIG coated on a 96-well plate. Standards and samples are pipetted into the wells and MIG present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIG bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Mouse MIG ELISA Kit (CXCL9) (ab203364) from our SimpleStep ELISA® range.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    240X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent C 1 x 30ml
    Biotinylated anti-mouse MIG 2 vials
    MIG Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse MIG Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 功能
    Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
  • 序列相似性
    Belongs to the intercrine alpha (chemokine CxC) family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • C-X-C motif chemokine 9
    • Chemokine (C-X-C motif) ligand 9
    • Chemokine, CXC motif, ligand 9
    • CMK
    • Crg 10
    • CXCL9
    • CXCL9_HUMAN
    • Gamma interferon induced monokine
    • Gamma-interferon-induced monokine
    • HuMIG
    • MIG
    • Monokine induced by gamma interferon
    • Monokine induced by interferon gamma
    • Monokine induced by interferon-gamma
    • SCYB9
    • Small inducible cytokine B9
    • Small inducible cytokine subfamily B member 9
    • Small-inducible cytokine B9
    see all
  • 数据库链接

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应用

Our Abpromise guarantee covers the use of ab100725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab100725

  • Representative standard curve using ab100725

实验方案

文献

ab100725 has not yet been referenced specifically in any publications.

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