小鼠MCP1 ELISA试剂盒(ab100722)
Key features and details
- Sensitivity: 3 pg/ml
- Range: 2.74 pg/ml - 2000 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠MCP1 ELISA试剂盒
参阅全部 MCP1 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture extracts, Tissue Extracts -
检测类型
Sandwich (quantitative) -
灵敏度
< 3 pg/ml -
范围
2.74 pg/ml - 2000 pg/ml -
回收率
86 %
特定样本回收率 样品类型 平均% 范围 Cell culture extracts 87.56 80% - 102% Tissue Extracts 85.89 78% - 101% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s MCP1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MCP1 in cell lysates and tissue lysates.
This assay employs an antibody specific for mouse MCP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 2X Cell Lysis Buffer 1 x 5ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml 5X Sample Diluent Buffer 1 x 10ml Biotinylated anti-Mouse MCP1 2 vials MCP1 Microplate (12 x 8 wells) 1 unit Recombinant Mouse MCP1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. -
序列相似性
Belongs to the intercrine beta (chemokine CC) family. -
翻译后修饰
Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. -
细胞定位
Secreted. - Information by UniProt
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别名
- C-C motif chemokine 2
- CCL2
- CCL2_HUMAN
see all -
数据库链接
- Entrez Gene: 20296 Mouse
- SwissProt: P10148 Mouse
- Unigene: 290320 Mouse
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab100722 被引用在 4 文献中.
- Li Z et al. A novel DPP-4 inhibitor Gramcyclin A attenuates cognitive deficits in APP/PS1/tau triple transgenic mice via enhancing brain GLP-1-dependent glucose uptake. Phytother Res 36:1297-1309 (2022). PubMed: 35088915
- Tang W et al. METTL3 enhances NSD2 mRNA stability to reduce renal impairment and interstitial fibrosis in mice with diabetic nephropathy. BMC Nephrol 23:124 (2022). PubMed: 35354439
- Hong YA et al. Extracellular Superoxide Dismutase Attenuates Renal Oxidative Stress Through the Activation of Adenosine Monophosphate-Activated Protein Kinase in Diabetic Nephropathy. Antioxid Redox Signal 28:1543-1561 (2018). PubMed: 29020797
- Coudriet GM et al. Treatment with a Catalytic Superoxide Dismutase (SOD) Mimetic Improves Liver Steatosis, Insulin Sensitivity, and Inflammation in Obesity-Induced Type 2 Diabetes. Antioxidants (Basel) 6:N/A (2017). Functional Studies ; Mouse . PubMed: 29104232