概述

  • 产品名称小鼠MCP1 ELISA试剂盒
    参阅全部 MCP1 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 3 pg/ml
  • 范围
    2.74 pg/ml - 2000 pg/ml
  • 复检

    94 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 95.29 84% - 104%
    Serum 94.28 83% - 103%
    Plasma 92.79 82% - 102%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s MCP1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MCP1 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse MCP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • 序列相似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻译后修饰Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • C-C motif chemokine 2
    • CCL2
    • CCL2_HUMAN
    • Chemokine (C C motif) ligand 2
    • GDCF 2
    • GDCF-2
    • GDCF2
    • HC11
    • HSMCR30
    • JE
    • MCAF
    • MCP 1
    • MCP-1
    • MCP1
    • MGC9434
    • Monocyte chemoattractant protein 1
    • Monocyte chemotactic and activating factor
    • Monocyte chemotactic protein 1
    • Monocyte secretory protein JE
    • SCYA2
    • Small inducible cytokine A2
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je)
    • Small inducible cytokine subfamily A (Cys Cys), member 2
    • Small-inducible cytokine A2
    • SMC CF
    • SMC-CF
    • SMCCF
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse MCP1 ELISA Kit 图像

  • MCP1 measured in mouse lysates showing quantity (pg) per microgram of total protein. Values retrieved from 1:100 and 1:1000 dilutions.

  • Representative standard curve using ab100721.

  • Representative standard curve using ab100721.

实验方案

Mouse MCP1 ELISA Kit (ab100721)参考文献

This product has been referenced in:
  • Lu HJ  et al. Ruscogenin ameliorates experimental nonalcoholic steatohepatitis via suppressing lipogenesis and inflammatory pathway. Biomed Res Int 2014:652680 (2014). Read more (PubMed: 25136608) »

See 1 Publication for this product

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