小鼠Lymphotactin ELISA试剂盒(SCM-1) (ab100720)

概述

  • 产品名称
    小鼠Lymphotactin ELISA试剂盒(SCM-1)
    参阅全部 Lymphotactin 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall < 10%
    批次间
    样品 n Mean SD CV%
    Overall < 12%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 40 pg/ml
  • 范围
    46.9 pg/ml - 3000 pg/ml
  • 复检

    100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 113.2 98% - 120%
    Serum 104.7 92% - 113%
    Plasma 94.64 83% - 128%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s Lymphotactin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of mouse Lymphotactin (SCM-1) in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for mouse Lymphotactin coated on a 96-well plate. Standards and samples are pipetted into the wells and Lymphotactin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Lymphotactin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Lymphotactin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent 1 x 15ml
    Biotinylated anti-Mouse Lymphotactin 2 vials
    Lymphotactin Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse Lymphotactin Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 功能
    Chemotactic activity for lymphocytes but not for monocytes or neutrophils.
  • 组织特异性
    Highest level in spleen, lower in peripheral leukocytes and very low levels in lung, colon and small intestine.
  • 序列相似性
    Belongs to the intercrine gamma family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • ATAC
    • C motif chemokine 1
    • Chemokine (C motif) ligand 1
    • Cytokine SCM-1
    • Cytokine SCM1
    • LPTN
    • LTN
    • Lymphotactin
    • Lymphotaxin
    • SCM 1
    • SCM 1 alpha
    • SCM-1-alpha
    • SCM1
    • SCM1 alpha
    • SCYC1
    • Small inducible cytokine C1
    • Small inducible cytokine subfamily C member 1
    • Small-inducible cytokine C1
    • XC chemokine ligand 1
    • Xcl1
    • XCL1_HUMAN
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative stadard curve using ab100720

实验方案

文献

ab100720 has not yet been referenced specifically in any publications.

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