小鼠lung组织裂解液- total protein (14 days) (ab7268)

概述

  • 产品名称
    小鼠lung组织裂解液- total protein (14 days)
    参阅全部 Lung 细胞裂解液
  • 描述
    小鼠lung总蛋白裂解液
  • 常规说明
    Source: Outbred NIH-Swiss Albino mice (14 days old)


    The mouse lung tissue was collected from fourteen (14) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • 经测试应用
    适用于: WBmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab7268 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use at an assay dependent dilution.
    Mouse lung tissue lysate is ready to load on SDS-PAGE for Western blotting. It is recommended to load 10 µg to 20 µg per lane for mini gel

    Mouse lung tissue lysate - total protein (14 days) (ab7268)参考文献

    This product has been referenced in:
    • Miller NR  et al. Sustained neuroprotection from a single intravitreal injection of PGJ2 in a nonhuman primate model of nonarteritic anterior ischemic optic neuropathy. Invest Ophthalmol Vis Sci 55:7047-56 (2014). Read more (PubMed: 25298416) »

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