小鼠liver组织裂解液- mitochondrial extract (ab110349)

概述

  • 产品名称小鼠liver组织裂解液- mitochondrial extract
    参阅全部 Mouse Liver 细胞裂解液
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.


    2 mg of mouse liver mitochondria in 400ul of iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE.
    1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.

  • 经测试应用适用于: SDS-PAGE, WBmore details

性能

  • 形式Liquid
  • 存放说明Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • 存储溶液Constituents: 0.02% PMSF, 0.16% Tris, 0.89% EDTA, 8.56% Sucrose
  • Concentration information loading...
  • 裂解物说明2 mg of mouse liver mitochondria in 400ul of iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE.
    1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.

    应用

    Our Abpromise guarantee covers the use of ab110349 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    SDS-PAGE Use at an assay dependent dilution.
    WB Use at an assay dependent dilution.

    Mouse liver tissue lysate - mitochondrial extract (ab110349)参考文献

    ab110349 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your phone call yesterday and for your patience while I have been in touch with the lab scientists at MitoSciences. Following is their reply to your questions: "These mitochondria were prepared by our mitochondrial isolation kit: http://w...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"