小鼠Lipocalin-2 ELISA试剂盒(NGAL) (ab119601)

概述

  • 产品名称小鼠Lipocalin-2 ELISA试剂盒(NGAL)
    参阅全部 Lipocalin-2 / NGAL 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    78 pg/ml - 5000 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s mouse Lipocalin-2 (NGAL) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Lipocalin-2 in cell culture supernatants, serum, plasma (heparin) and urine.

    A Lipocalin-2 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Lipocalin-2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Lipocalin-2 amount of sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
  • 组织特异性Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
  • 序列相似性Belongs to the calycin superfamily. Lipocalin family.
  • 细胞定位Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
  • Information by UniProt
  • 别名
    • 24p3
    • 25 kDa alpha-2-microglobulin-related subunit of MMP-9
    • HNL
    • Lcn2
    • Lipocalin 2
    • Lipocalin-2
    • Migration stimulating factor inhibitor
    • MSFI
    • Neutrophil gelatinase-associated lipocalin
    • NGAL
    • NGAL_HUMAN
    • Oncogene 24p3
    • p25
    • Siderocalin
    • Siderocalin LCN2
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse Lipocalin-2 ELISA Kit (NGAL) 图像

  • Lipocalin-2 measured in biological fluids (dilution range 1/50-1/500; duplicates +/- SD).

  • Mouse lipocalin-2 measured in tissue lysates (10-50 ug protein per mL tested; presented as NGAL per mg of extracted protein; duplicates +/- SD).

  • Lipocalin-2 measured in culture supernatants (dilution range 1/1-1/25) from control (-) or activated J774 cells ((PHA) 48 hours with 2% PHA-M; LifeTechnologies) and from RAW264 cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) with the addition of 1 ug x mL-1 of LPS (Sigma) for the last 6 hours (PMA/LPS) (duplicates +/- SD).

  • Representative Standard Curve using ab119601

实验方案

Mouse Lipocalin-2 ELISA Kit (NGAL) (ab119601)参考文献

ab119601 has not yet been referenced specifically in any publications.

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