小鼠Interferon gamma ELISA试剂盒(ab46081)

概述

  • 产品名称小鼠Interferon gamma ELISA试剂盒
    参阅全部 Interferon gamma 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    A 20 315.4pg/ml 14.5 4.6%
    B 19 588.6pg/ml 19.1 4.6%
    批次间
    样品 n Mean SD CV%
    A 9 128.8pg/ml 7.4 5.7%
    B 10 546.9pg/ml 25.2 4.6%
  • 测试
    1 x 96 well plate
  • 样品类型
    Milk, Serum
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 15 pg/ml
  • 范围
    31.25 pg/ml - 1000 pg/ml
  • 检测时间
    3h 45m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s IFN gamma mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IFN gamma in mouse serum, buffered solutions and cell culture medium.

    A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples.

    This kit will recognize both endogenous and recombinant mouse IFN gamma.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
  • 别名
    • IFG
    • IFI
    • IFN gamma
    • IFN Immune
    • IFNG
    • Interferon gamma
    • Interferon Gamma Precursor
    • Macrophage Activating Factor
    • MAF
    • T Cell Interferon
    • Type II Interferon
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse Interferon gamma ELISA Kit 图像

  •  Representative Standard Curve using ab46081

实验方案

Mouse Interferon gamma ELISA Kit (ab46081)参考文献

ab46081 has not yet been referenced specifically in any publications.

Product Wall

Gracias por contactarnos.


He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

Tenemos varios kits contra dichas citoquinas espec&iacu...

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Unfortunately, this kit is a ELISpot assay dedicated to detect IFNg secreting cells and not appropriate for serum.

This product will work for your customer's samples:

http://www.abcam.com/index.html?datasheet=46081 (or use ...

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Thank you for contacting us.

The HRP-streptavidin is available in catalogue. Please buy the product ab96467.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or informatio...

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Thank you for contacting us. We still had your email address wrong. Now it is fine, you should receiving my emails.

To let you know I have requested information from the lab regarding the HRP-strepatavidin conjugateand now waiting a reply fr...

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I have requested information from the lab and now waiting a reply.

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Thank you for contacting us.

I have had the chance to speak with the lab regarding your inquiry.Regrettably, the standards includedwithin the Interferon gamma Mouse ELISA kit are not correlated with Biological activity Unit; these tests ha...

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Abreview for Interferon gamma Mouse ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Mouse Serum (Plasma)
Specification: Plasma
Amount of Sample: 10 µg
Type of Kit Used: ELISA
Positive control: C57Bl/6 male mice 8 weeks of age were treated with LPS IP 1mg/kg (0111:B4). After 4 hours blood was collected in EDTA coated tubes. 1:10 dilutions of plasma were made with standard dilutend and used for ELISA. All samples (1:10 diluted) were in range of the standard curve.
Did you use the Abcam Kit protocol?: Yes
Username

Mr. Thomas Milsom

Verified customer

提交于 Feb 24 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"