小鼠IL2酶联免疫斑点法(ELISPOT)试剂盒(ab64031)

概述

  • 产品名称
    小鼠IL2酶联免疫斑点法(ELISPOT)试剂盒
    参阅全部 IL2 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Cell culture extracts
  • 检测类型
    Sandwich (qualitative)
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.

    Abcam Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

    Principal

    After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.

     

    Recognizes natural murine IL2

  • 经测试应用
    适用于: ELISpotmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 标识符 1 x 96 tests 5 x 96 tests
    Bovine Serum Albumin 1 x 2g
    IL2 Biotinylated Detection antibody 1 x 120µl
    Mouse IL2 Pre-coated 96-well PDVF-bottomed plates 2 units
    Ready-to-use BCIP/NBT substrate buffer 1 x 27ml
    Streptavidin - Alkaline Phosphatase conjugated 1 x 15µl
  • 研究领域
  • 相关性
    Human interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.
  • 细胞定位
    Secreted
  • 别名
    • Aldesleukin
    • IL2
    • Interleukin 2
    • Lymphokine
    • T Cell Growth Factor
    • TCGF
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab64031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISpot Use at an assay dependent dilution.

文献

ab64031 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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