小鼠IL-7 ELISA试剂盒(Interleukin-7) (ab100714)

概述

  • 产品名称
    小鼠IL-7 ELISA试剂盒(Interleukin-7)
    参阅全部 IL7 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    8.23 pg/ml - 6000 pg/ml
  • 复检

    93 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 94.58 85% - 105%
    Serum 91.59 82% - 103%
    Plasma 92.66 83% - 104%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s IL-7 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-7 in serum, plasma and cell culture supernates.

    This assay employs an antibody specific for mouse IL-7 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    440X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-mouse IL-7 2 vials
    IL-7 Microplate (12 x 8 wells) 1 unit
    Recombinant mouse IL-7 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 相关性
    IL7 is a cytokine important for B and T cell development. It and the hepatocyte growth factor form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL7 is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. It can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that IL7 plays an essential role in lymphoid cell survival.
  • 细胞定位
    Secreted
  • 别名
    • IL 7
    • IL7 nirs variant 6
    • Interleukin 7
    • Interleukin7
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab100714

  • Representative standard curve using ab100714

实验方案

文献

ab100714 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting Abcam regarding ab100714.


The IL7 Mouse ELISA Kit will detect soluble IL-7. As this kit was not validated with cell or tissue lysate samples, we do not know if the kit will also detect IL-7 bound to its membrane...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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