小鼠IL-6 ELISA试剂盒(Interleukin-6) (ab100713)

概述

  • 产品名称小鼠IL-6 ELISA试剂盒(Interleukin-6)
    参阅全部 IL6 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture extracts, Tissue Extracts
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 2 pg/ml
  • 范围
    0.82 pg/ml - 600 pg/ml
  • 复检

    93 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture extracts 94.53 84% - 104%
    Tissue Extracts 93.39 83% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s IL-6 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-6 in cell lysates and tissue lysates.

    This assay employs an antibody specific for mouse IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • 疾病相关Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • 序列相似性Belongs to the IL-6 superfamily.
  • 翻译后修饰N- and O-glycosylated.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • B cell differentiation factor
    • B cell stimulatory factor 2
    • B-cell stimulatory factor 2
    • BSF 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • Cytotoxic T cell differentiation factor
    • Hepatocyte stimulating fact
    • HGF
    • HPGF
    • HSF
    • Hybridoma growth factor
    • Hybridoma plasmacytoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL 6
    • IL-6
    • IL6
    • IL6 protein
    • IL6_HUMAN
    • Interferon beta 2
    • Interferon beta-2
    • Interleukin 6
    • Interleukin 6 (interferon beta 2)
    • Interleukin BSF 2
    • Interleukin-6
    • Interleukin6
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-6 ELISA Kit (Interleukin-6) 图像

  • IL-6 measured in mouse tissue lysates prepared with RIPA buffer and sonicated in an ice-cold water bath for 12 intervals of a total of 3.5 minutes. 0.02-1 mg protein x mL-1 were tested, results shown as IL-6 per mg of extracted protein (duplicates +/- SD).

  • IL-6 measured in RIPA lysates from control cells, or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297), with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours. Samples were diluted and tested in the range of 1e4-5e5 cells x mL-1, results expressed per million cells; duplicates +/- SD).

  • Dilution curves of mouse IL-6 (ab100713) and NIBSC standard (93/730). One pg of standard mouse IL-6 corresponds to 0.37 (+/- 0.07) AU. Background signal subtracted (duplicates; +/- SD).

  • Standard curve of mouse IL-6 with background signal subtracted (duplicates; +/- SD).

  • Representative standard curve using ab100713

实验方案

Mouse IL-6 ELISA Kit (Interleukin-6) (ab100713)参考文献

This product has been referenced in:
  • Pereira DM  et al. Anti-inflammatory effect of unsaturated fatty acids and Ergosta-7,22-dien-3-ol from Marthasterias glacialis: prevention of CHOP-mediated ER-stress and NF-?B activation. PLoS One 9:e88341 (2014). Read more (PubMed: 24551093) »
  • Hsu TM  et al. Effects of sucrose and high fructose corn syrup consumption on spatial memory function and hippocampal neuroinflammation in adolescent rats. Hippocampus N/A:N/A (2014). Read more (PubMed: 25242636) »

See all 4 Publications for this product

Product Wall

Thank you for getting in touch.

We have not tested ab100713 Mouse IL-6 ELISA Kit (Interleukin-6) specifically in liver samples in our own laboratories. However, I am happy to provide you with some general guidelines for tissue lysate preparati...

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IL6 in Rat liver tissue

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Abreviews
The standard curve was as described as the typical curve. One concentration had to be eliminated to give a high R^2 value.
Samples were from rat (normal out-bred sprague dawley from Harlan) liver tissue, homogenized using a 1% np-40 buffer. Although a 1:10 dilution was used for the samples (which was sufficiently in range of the IL1b ELISA (ab100768) standards), the absorbances were out of range of the standards.
For the samples that did fall in the standard range, the levels of IL6 in control rats seem to be a little higher (~225-325pg/ml) than the expected range (~125-200pg/ml).
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提交于 Apr 02 2014

Thank you for your reply.

Just to clarify, the diluted/working standards (600, 200, 66.7, 22.2, 7.4, 2.5, 0.82 pg/ml) should not be stored. The reconstituted standard (50 ng/ml) can be stored at -80ºC for 1-2 weeks at most. Remember, an ...

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Thank you for your inquiry.

We know of the IL6 ELISA kit being tested in liver tissue lysate, but unfortunately we do not have any info on brain lysate.

For the SDF1 alpha ELISA kit, we haven't tested tissues with this kit but it wou...

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Thank you for contacting us.

The following ELISA kits for mouse ICAM, VCAM, IL-6 and TNF-alpha are suitable for mouse samples:

ab100688 (http://www.abcam.com/ICAM1-Mouse-ELISA-Kit-1-x-96-Well-Plate-ab100688.html)
ab100713 (htt...

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Thank you for contacting us this afternoon.

I have been to have a look at how the IL6 Mouse ELISA Kit (ab100713) looks and to take photos so that you could get a better idea of how it would work. The plate itself is a 96 well plate but can be...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"