小鼠IL-6 ELISA试剂盒(Interleukin-6) (ab100712)

概述

  • 产品名称
    小鼠IL-6 ELISA试剂盒(Interleukin-6)
    参阅全部 IL6 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma, Tissue Lysate
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 2 pg/ml
  • 范围
    0.82 pg/ml - 600 pg/ml
  • 复检

    94 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 96.18 86% - 103%
    Serum 93.39 83% - 103%
    Plasma 94.53 84% - 104%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
    预测可用于: Human
  • 产品概述

    Abcam’s IL-6 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-6 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent 1 x 15ml
    Biotinylated anti-mouse IL-6 2 vials
    IL-6 Microplate (12 x 8 wells) 1 unit
    Recombinant mouse IL-6 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 功能
    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • 疾病相关
    Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • 序列相似性
    Belongs to the IL-6 superfamily.
  • 翻译后修饰
    N- and O-glycosylated.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • B cell differentiation factor
    • B cell stimulatory factor 2
    • B-cell stimulatory factor 2
    • BSF 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • Cytotoxic T cell differentiation factor
    • Hepatocyte stimulating fact
    • HGF
    • HPGF
    • HSF
    • Hybridoma growth factor
    • Hybridoma plasmacytoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL 6
    • IL-6
    • IL6
    • IL6 protein
    • IL6_HUMAN
    • Interferon beta 2
    • Interferon beta-2
    • Interleukin 6
    • Interleukin 6 (interferon beta 2)
    • Interleukin BSF 2
    • Interleukin-6
    • Interleukin6
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Standard curve of msIL-6 in different diluents with background signal subtracted (duplicates; +/- SD).
  • IL-6 measured in mouse tissue lysates (expressed as per mg of extracted protein), with background signal subtracted (duplicates; +/- SD).
  • msIL-6 measured in biological fluids, background signal subtracted (duplicates +/- SD).
  • IL-6 detected in supernatants from RAW 246.7 control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), or 24 hours with PMA and 1 ug x mL-1 of LPS (Sigma) (P+L) for the last 6 hours. Results shown after background signal was subtracted (duplicates +/- SD).
  • Representative standard curve using ab100712

  • Representative standard curve using ab100712

实验方案

文献

This product has been referenced in:
  • Al-Muzafar HM & Amin KA Probiotic mixture improves fatty liver disease by virtue of its action on lipid profiles, leptin, and inflammatory biomarkers. BMC Complement Altern Med 17:43 (2017). Rat . Read more (PubMed: 28086768) »
  • Zheng J  et al. Maternal Low-Protein Diet Modulates Glucose Metabolism and Hepatic MicroRNAs Expression in the Early Life of Offspring †. Nutrients 9:N/A (2017). ELISA ; Mouse . Read more (PubMed: 28264458) »

See all 7 Publications for this product

客户评价及客户问答

Gracias por tu respuesta.

No hay ningún problema en reemplazarte el kit al encontrarse éste en garantía. El nuevo pedido gratuito tiene referencia XXXX.

Te llegará un mail de confirmación del nuevo...

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Te contacto de nuevo porque parece que hemos encontrado la fuente del error.

En el protocolo hay una incongruencia a la hora de especificar la dilución del vial de HRP Streptavidina. Primero indica que debe diluirse 10,000 veces en Ass...

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Te pido disculpas por la espera.

Para tratar de entender los resultados obtenidos estoy comentando con mis compañeros el protocolo seguido, y así asegurar que no volvéis a tener ningún problema con este kit.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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