小鼠IL-5 ELISA试剂盒(Interleukin-5) (ab100711)

概述

  • 产品名称小鼠IL-5 ELISA试剂盒(Interleukin-5)
    参阅全部 IL5 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 1 pg/ml
  • 范围
    2.05 pg/ml - 500 pg/ml
  • 复检

    94 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 95.76 85% - 104%
    Serum 93.44 82% - 102%
    Plasma 95.49 93% - 104%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s IL-5 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-5 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse IL-5 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
  • 序列相似性Belongs to the IL-5 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • B cell differentiation factor I
    • B-cell differentiation factor I
    • Colony stimulating factor eosinophil
    • EDF
    • Eosinophil differentiation factor
    • IL 5
    • IL-5
    • IL5
    • IL5_HUMAN
    • Interleukin 5
    • Interleukin 5 (colony stimulating factor eosinophil)
    • Interleukin-5
    • Interleukin5
    • T cell replacing factor
    • T-cell replacing factor
    • TRF
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-5 ELISA Kit (Interleukin-5) 图像

  • Representative standard curve using ab100711

  • Representative standard curve using ab100711

实验方案

Mouse IL-5 ELISA Kit (Interleukin-5) (ab100711)参考文献

ab100711 has not yet been referenced specifically in any publications.

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