小鼠IL-4 ELISA试剂盒(Interleukin-4) (ab100710)

概述

  • 产品名称小鼠IL-4 ELISA试剂盒(Interleukin-4)
    参阅全部 IL4 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture extracts, Tissue Extracts
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 1 pg/ml
  • 范围
    0.819 pg/ml - 200 pg/ml
  • 复检

    92 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture extracts 93.62 84% - 104%
    Tissue Extracts 91.57 82% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s Mouse IL-4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-4 in cell lysates and tissue lysates.

    This assay employs an antibody specific for mouse IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性IL4 is a pleiotropic cytokine produced by activated T cells. It is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of IL4. IL4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of IL4 encoding distinct isoforms have been reported.
  • 细胞定位Secreted
  • 别名
    • B cell growth factor 1
    • B cell stimulatory factor 1
    • BCGF 1
    • BCGF1
    • Binetrakin
    • BSF 1
    • BSF1
    • IL 4
    • Interleukin 4
    • Lymphocyte stimulatory factor 1
    • MGC79402
    • Pitrakinra
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-4 ELISA Kit (Interleukin-4) 图像

  • Representative standard curve using ab100710

实验方案

Mouse IL-4 ELISA Kit (Interleukin-4) (ab100710)参考文献

ab100710 has not yet been referenced specifically in any publications.

Product Wall

I have broken down the cross-reactivity information for each kit below:


· ab100549 IL10 Human ELISA Kit
o The capture antibody did not show any cross-reactivity with Murine IL-10 from testing. Thus, this kit is not expected ...

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Thank you for your inquiry.

I can confirm that the sample diluent buffer would be the most suitable buffer to dilute the serum samples.

I suggest to do a serial dilution to find the optimal dilution.

I hope this informatio...

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Thank you for your inquiry.

I can confirm that these kit are suitable for the use with serum samples that have been frozen.

I recommend to not to mix/compare samples thathave been frozen with samples that have not been frozen unless...

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Thank you for your inquiry. I can confirm that all these kits are suitable for mouse samples: ab83362 and ab102512: These kits can be used with a variety of samples such as serum, plasma, and urine and should not be species dependent. As long as there ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"