小鼠IL-4 ELISA试剂盒(ab100710)
Key features and details
- Sensitivity: 1 pg/ml
- Range: 0.819 pg/ml - 200 pg/ml
- Sample type: Cell Lysate, Tissue Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠IL-4 ELISA试剂盒
参阅全部 IL-4 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell Lysate, Tissue Lysate -
检测类型
Sandwich (quantitative) -
灵敏度
< 1 pg/ml -
范围
0.819 pg/ml - 200 pg/ml -
回收率
92 %
特定样本回收率 样品类型 平均% 范围 Tissue Extracts 91.57 82% - 103% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s Mouse IL-4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-4 in cell lysates and tissue lysates.
This assay employs an antibody specific for mouse IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 2X Cell Lysis Buffer 1 x 5ml 5X Assay Diluent 1 x 15ml 5X Sample Diluent Buffer 1 x 10ml 600X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated anti-Mouse IL-4 2 vials IL-4 Microplate (12 x 8 wells) 1 unit Recombinant mouse IL-4 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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相关性
IL-4 is a pleiotropic cytokine produced by activated T cells. It is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of IL-4. IL-4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL-4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of IL-4 encoding distinct isoforms have been reported. -
细胞定位
Secreted -
别名
- B cell growth factor 1
- B cell stimulatory factor 1
- BCGF 1
see all -
数据库链接
- Entrez Gene: 16189 Mouse
- SwissProt: P07750 Mouse
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab100710 被引用在 21 文献中.
- Lo YT et al. Immunogenicity of a secreted, C-terminally truncated, form of bovine viral diarrhea virus E2 glycoprotein as a potential candidate in subunit vaccine development. Sci Rep 13:296 (2023). PubMed: 36609424
- Khan MA et al. Liposome-Mediated Delivery of MERS Antigen Induces Potent Humoral and Cell-Mediated Immune Response in Mice. Molecules 27:N/A (2022). PubMed: 35056718
- Xiang J et al. Lidocaine relieves murine allergic rhinitis by regulating the NF-κB and p38 MAPK pathways. Exp Ther Med 23:193 (2022). PubMed: 35126696
- Song J & Wang J SIRT3 regulates bronchial epithelium apoptosis and aggravates airway inflammation in asthma. Mol Med Rep 25:N/A (2022). PubMed: 35234263
- Xiu W et al. Potentiating hypoxic microenvironment for antibiotic activation by photodynamic therapy to combat bacterial biofilm infections. Nat Commun 13:3875 (2022). PubMed: 35790729