小鼠IL-3 ELISA试剂盒(Interleukin-3) (ab113345)

概述

  • 产品名称小鼠IL-3 ELISA试剂盒(Interleukin-3)
    参阅全部 IL3 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 0.2 pg/ml
  • 范围
    0.16 pg/ml - 40 pg/ml
  • 复检

    > 100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 102.4 68% - 132%
    Serum 116.2 72% - 131%
    Plasma 121.9 85% - 142%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s IL-3 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-3 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse IL-3 coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
    This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
  • 组织特异性Activated T-cells, mast cells, natural killer cells.
  • 序列相似性Belongs to the IL-3 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Burst promoting activity
    • Colony stimulating factor multiple
    • Eosinophil colony stimulating factor
    • Hematopoietic growth factor
    • IL 3
    • IL-3
    • IL3
    • IL3_HUMAN
    • Interleukin 3
    • Interleukin-3
    • Mast cell growth factor
    • MCGF
    • Multi CSF
    • Multilineage colony stimulating factor
    • Multipotential colony stimulating factor
    • Multipotential colony-stimulating factor
    • P cell stimulating factor
    • P-cell-stimulating factor
    • Thy1 inducing factor
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab113345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse IL-3 ELISA Kit (Interleukin-3) 图像

  • Representative Standard Curve using ab113345.

实验方案

Mouse IL-3 ELISA Kit (Interleukin-3) (ab113345)参考文献

ab113345 has not yet been referenced specifically in any publications.

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