小鼠IL-23 ELISA试剂盒(Interleukin-23) (ab119545)

概述

  • 产品名称
    小鼠IL-23 ELISA试剂盒(Interleukin-23)
    参阅全部 IL23 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 8.3%
    批次间
    样品 n Mean SD CV%
    Overall 2.5%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    16 pg/ml
  • 范围
    31.3 pg/ml - 2000 pg/ml
  • 复检

    特定样本复检结果
    样品类型 平均% 范围
    Serum 49 41% - 53%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s IL-23 (Interleukin-23) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-23 in cell culture supernatant, serum and plasma (citrate).

    IL-23 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated IL-23 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-23 captured on the plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Biotin-Conjugate anti-Mouse IL-23 monoclonal antibody 1 x 70µl
    IL-23 Standard lyophilized (4,000 pg/mL upon reconstitution) 2 vials
    Microplate coated with monoclonal antibody to Mouse IL-23 (12 x 8 wells) 1 unit
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • 研究领域
  • 功能
    Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • 组织特异性
    Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).
  • 序列相似性
    Belongs to the IL-6 superfamily.
  • 发展阶段
    Expressed by newborns dendritic cells.
  • 细胞定位
    Secreted. Secreted upon association with IL12B.
  • Information by UniProt
  • 别名
    • IL 23
    • IL 23A
    • IL-23 subunit alpha
    • IL-23-A
    • IL-23p19
    • Il23a
    • IL23A_HUMAN
    • IL23P19
    • Interleukin 23 alpha subunit p19
    • Interleukin-23 subunit alpha
    • Interleukin-23 subunit p19
    • JKA3 induced upon T cell activation
    • P19
    • SGRF
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab119545.

     

实验方案

文献

ab119545 has not yet been referenced specifically in any publications.

客户评价及客户问答



Ab119545 has only been tested with culture supernatant, serum, and citrate plasma, but it may be suitable for use with untested sample types like tissue lysates.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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